{"id":"W2011379101","doi":"10.1002/prot.22079","title":"Tethering polypeptides through bifunctional PEG cross‐linking agents to probe protein function: Application to ATP synthase","year":2008,"lang":"en","type":"article","venue":"Proteins Structure Function and Bioinformatics","topic":"ATP Synthase and ATPases Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Schulich School of Medicine and Dentistry; Schulich School of Medicine and Dentistry, Western University; University of Oregon","keywords":"ATP synthase; PEG ratio; Bifunctional; Linker; Biochemistry; Chemistry; Enzyme; Protein subunit; Biophysics; ATP hydrolysis; ATPase; Cysteine; Molecular motor; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0001363267,0.0002932376,0.00018883,0.0001200463,0.0007422659,0.0001402604,0.0001617519,0.0002257148,0.0001011048],"category_scores_gemma":[0.0001017692,0.0002516202,0.00008061832,0.0003439937,0.0001229208,0.00005786687,0.000212618,0.0002086489,0.00006817522],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004336136,"about_ca_system_score_gemma":0.000124918,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003985288,"about_ca_topic_score_gemma":0.00002264428,"domain_scores_codex":[0.9983294,0.00003083482,0.0004119066,0.0004096478,0.0004208387,0.0003973959],"domain_scores_gemma":[0.9990191,0.00000789994,0.0001278147,0.0003883017,0.0002170526,0.0002398297],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001318296,0.00009488842,0.004636368,0.0003519931,0.0001508507,0.000003275888,0.0007769443,0.0009174265,0.9481436,0.0005201633,0.001113139,0.04197307],"study_design_scores_gemma":[0.00278111,0.004236241,0.02932605,0.0002843922,0.00008750581,0.0001852338,0.0008438777,0.004033838,0.4820309,0.001280221,0.4731628,0.001747828],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5986817,0.00007201851,0.3987587,0.0002651956,0.0001583183,0.001527973,0.00006096402,0.00005995019,0.0004151961],"genre_scores_gemma":[0.9663507,0.0000158219,0.03023528,0.001248024,0.000596137,0.0003319335,0.0002783308,0.0000375319,0.0009062104],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.4720497,"threshold_uncertainty_score":0.9999936,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01832220194101373,"score_gpt":0.277279353216321,"score_spread":0.2589571512753072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}