{"id":"W2011379101","doi":"10.1002/prot.22079","title":"Tethering polypeptides through bifunctional PEG cross‐linking agents to probe protein function: Application to ATP synthase","year":2008,"lang":"en","type":"article","venue":"Proteins Structure Function and Bioinformatics","topic":"ATP Synthase and ATPases Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":1,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Schulich School of Medicine and Dentistry; Schulich School of Medicine and Dentistry, Western University; University of Oregon","keywords":"ATP synthase; PEG ratio; Bifunctional; Linker; Biochemistry; Chemistry; Enzyme; Protein subunit; Biophysics; ATP hydrolysis; ATPase; Cysteine; Molecular motor; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001678621,0.0003398397,0.0001176349,0.0001390636,0.00008737209,0.0001818112,0.0001933094,0.0004370241,0.0003896298],"category_scores_gemma":[0.0001185747,0.0001146311,0.000116357,0.0001133591,0.0001691746,0.0002289258,0.0001696527,0.0004475893,0.0001520222],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002505625,"about_ca_system_score_gemma":0.00009480029,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000265223,"about_ca_topic_score_gemma":0.0002579575,"domain_scores_codex":[0.9999399,0.00001108359,0.000004821585,0.00002068258,0.00001358521,0.00001000141],"domain_scores_gemma":[0.9999368,0.00001768349,0.00001527765,0.000008163763,0.000007163853,0.00001487671],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000006137444,0.000004747315,0.00002317677,0.0000140827,9.684414e-7,0.00001082559,0.000004012781,0.00003256019,0.999151,0.0000512933,0.000005153358,0.0006958905],"study_design_scores_gemma":[0.000002337725,0.00003897012,0.0002895799,0.000001746155,0.000003308662,0.00006561426,0.000003290072,0.0005907585,0.9982395,0.00002066283,0.0007426295,0.0000015171],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9498156,0.001822916,0.04683379,0.0001660558,0.00002521599,0.00005736405,0.0001168454,0.0001663814,0.0009958571],"genre_scores_gemma":[0.9671079,0.002141479,0.02898861,0.00007980621,0.000007648098,0.00004875014,0.0002099954,0.00003178188,0.001384007],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0004370241,"threshold_uncertainty_score":0.001817942,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01832220194101373,"score_gpt":0.277279353216321,"score_spread":0.2589571512753072,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}