{"id":"W2011577522","doi":"10.1016/j.vascn.2010.03.002","title":"Principles and problems of the electrophoretic mobility shift assay","year":2010,"lang":"en","type":"article","venue":"Journal of Pharmacological and Toxicological Methods","topic":"NF-κB Signaling Pathways","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"","keywords":"Electrophoretic mobility shift assay; DNA; Molecular biology; Polyacrylamide gel electrophoresis; Gel electrophoresis; Lysis; Biochemistry; DNA-binding protein; Chemistry; Electrophoresis; Biology; Gene expression; Enzyme; Gene; Transcription factor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.01222903,0.002656366,0.003845231,0.00645512,0.00241881,0.006605964,0.007155833,0.004784116,0.001664749],"category_scores_gemma":[0.01610553,0.002938741,0.001663629,0.003005396,0.01529619,0.007542717,0.00447169,0.01352449,0.003500132],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00260899,"about_ca_system_score_gemma":0.002317282,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009801175,"about_ca_topic_score_gemma":0.0005023769,"domain_scores_codex":[0.9886075,0.004658679,0.0008891163,0.001926377,0.003551717,0.0003667642],"domain_scores_gemma":[0.9850078,0.009770924,0.0006631144,0.002751932,0.001497847,0.0003084809],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"theoretical_or_conceptual","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002434201,0.0004325311,0.0009194424,0.001380387,0.0001185102,0.0005751979,0.0009586664,0.004742112,0.08570363,0.7633595,0.01155386,0.1300126],"study_design_scores_gemma":[0.00006130851,0.0002554947,0.0008589076,0.0002917837,0.00006221041,0.002520673,0.0002180212,0.02784072,0.06069004,0.8030167,0.1037855,0.0003986581],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.002168648,0.01373061,0.9749509,0.003819052,0.000927909,0.00009817646,0.0001874144,0.000540394,0.003576915],"genre_scores_gemma":[0.05300289,0.02911054,0.9050944,0.002448545,0.001929383,0.00174126,0.0003805785,0.0002591261,0.006033272],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01222903,"threshold_uncertainty_score":0.06467402,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03741783088277189,"score_gpt":0.3583204836894731,"score_spread":0.3209026528067012,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}