{"id":"W2012250005","doi":"10.1385/mb:34:2:225","title":"Large-Scale Transfection of Mammalian Cells for the Fast Production of Recombinant Protein","year":2006,"lang":"en","type":"review","venue":"Molecular Biotechnology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":238,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute; National Research Council Canada; Cegep de Saint Hyacinthe","funders":"","keywords":"Chinese hamster ovary cell; Transfection; Recombinant DNA; Biology; Cloning (programming); Complementary DNA; Baby hamster kidney cell; HEK 293 cells; Polyethylenimine; Cell biology; Protein engineering; Molecular biology; Computational biology; Gene; Cell culture; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000263993,0.0003135429,0.0007282165,0.000174375,0.00005749632,0.000005857022,0.0004529679,0.00109967,0.000003736454],"category_scores_gemma":[0.0000366169,0.0002331499,0.0006140971,0.0002461429,0.000203427,0.000002039762,0.0000886686,0.0002249961,0.000002985865],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001830283,"about_ca_system_score_gemma":0.0001052247,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000396897,"about_ca_topic_score_gemma":0.00008954468,"domain_scores_codex":[0.9983773,0.00009531502,0.0005918216,0.0005459141,0.0001023719,0.0002872802],"domain_scores_gemma":[0.9987076,0.000007747298,0.0003453538,0.0007927555,0.0001279259,0.00001863676],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003764063,0.0001602171,2.557146e-7,0.003514175,0.0001956986,0.000001483458,0.000009271225,0.00001904482,0.7149627,0.0001141716,0.0006765492,0.2803088],"study_design_scores_gemma":[0.0001156957,0.0004709489,2.287503e-7,0.0004165408,0.0002067639,0.00001385488,0.00001951409,0.000003840512,0.544233,0.00003015114,0.4543563,0.0001331744],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0076472,0.9577889,0.03098608,0.0001687099,0.0007245408,0.002526985,0.0001135693,0.00002101927,0.00002301963],"genre_scores_gemma":[0.007454141,0.9897149,0.0007434893,0.00001194987,0.0001023099,0.0005369259,0.0003798181,0.00007290218,0.0009836302],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.4536798,"threshold_uncertainty_score":0.9507577,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01158562254988946,"score_gpt":0.2604807559889509,"score_spread":0.2488951334390615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}