{"id":"W2012250005","doi":"10.1385/mb:34:2:225","title":"Large-Scale Transfection of Mammalian Cells for the Fast Production of Recombinant Protein","year":2006,"lang":"en","type":"review","venue":"Molecular Biotechnology","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":238,"is_retracted":false,"has_abstract":false,"ca_institutions":"Biotechnology Research Institute; National Research Council Canada; Cegep de Saint Hyacinthe","funders":"","keywords":"Chinese hamster ovary cell; Transfection; Recombinant DNA; Biology; Cloning (programming); Complementary DNA; Baby hamster kidney cell; HEK 293 cells; Polyethylenimine; Cell biology; Protein engineering; Molecular biology; Computational biology; Gene; Cell culture; Biochemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001317572,0.002364018,0.00311718,0.001944759,0.0004393615,0.001319126,0.002283559,0.00153151,0.002288012],"category_scores_gemma":[0.0005639623,0.0006781249,0.0008090829,0.002372165,0.0009765425,0.001293955,0.0008573605,0.002741466,0.006226123],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001120587,"about_ca_system_score_gemma":0.0007976993,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007122994,"about_ca_topic_score_gemma":0.001131667,"domain_scores_codex":[0.9994357,0.00007975143,0.00004562702,0.00009586413,0.0002927099,0.0000502821],"domain_scores_gemma":[0.9996678,0.0001385354,0.00005156213,0.000035491,0.00007588899,0.00003073054],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001734938,0.0002241856,0.0001530534,0.008161533,0.00008978995,0.0004888094,0.0001182234,0.0009508215,0.2995185,0.01042739,0.02407219,0.6556219],"study_design_scores_gemma":[0.00007083992,0.0002325438,0.000740988,0.0005122743,0.0001491617,0.002622495,0.00004423534,0.0009803848,0.1787769,0.003463277,0.8123395,0.00006730985],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.003074417,0.9052007,0.08068896,0.0007958252,0.001938123,0.0001927668,0.000317955,0.0007371693,0.007054135],"genre_scores_gemma":[0.01085543,0.9258128,0.0494106,0.0005864467,0.0004529181,0.000305993,0.001353526,0.0001675316,0.01105465],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.00311718,"threshold_uncertainty_score":0.008130431,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01158562254988946,"score_gpt":0.2604807559889509,"score_spread":0.2488951334390615,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}