{"id":"W2012257747","doi":"10.1371/journal.pone.0088464","title":"2PE-STED Microscopy with a Single Ti: Sapphire Laser for Reduced Illumination","year":2014,"lang":"en","type":"article","venue":"PLoS ONE","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Program for New Century Excellent Talents in University; National Natural Science Foundation of China","keywords":"STED microscopy; Sapphire; Laser; Optics; Stimulated emission; Materials science; Microscopy; Fluorescence microscope; Ti:sapphire laser; Super-resolution microscopy; Optoelectronics; Fluorescence; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003070617,0.0005461091,0.000495052,0.0003086145,0.000231997,0.0002887207,0.0007426395,0.0006157051,0.0009483176],"category_scores_gemma":[0.0002011002,0.0003232306,0.0003130877,0.0001708967,0.0002794043,0.0003936859,0.0006525833,0.0008758202,0.0004748309],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002546386,"about_ca_system_score_gemma":0.000259375,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003240989,"about_ca_topic_score_gemma":0.0007271271,"domain_scores_codex":[0.9997513,0.00002774694,0.0000157716,0.00006536512,0.000103735,0.00003617662],"domain_scores_gemma":[0.9999107,0.00002814015,0.00001157182,0.00002282435,0.00001646675,0.0000102558],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001385697,0.00000641907,0.00004078729,0.00003460763,0.000002829374,0.00003955125,0.000008770951,0.0001282472,0.9973395,0.0002436567,0.0000380801,0.0021036],"study_design_scores_gemma":[0.000008069787,0.00004420177,0.0003057753,0.000003449834,0.000005689735,0.0001927506,0.00000521568,0.006843497,0.9909983,0.0001166959,0.001467245,0.000009215661],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6848521,0.001776785,0.3074261,0.000442874,0.0001997248,0.0001516658,0.0005146354,0.0008443714,0.003791814],"genre_scores_gemma":[0.6699158,0.001103251,0.3247192,0.0001122364,0.00005205162,0.0002287523,0.000429387,0.000110554,0.003328717],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009483176,"threshold_uncertainty_score":0.003172398,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01850242649387277,"score_gpt":0.2506679905332733,"score_spread":0.2321655640394006,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}