{"id":"W201238165","doi":"","title":"Optimization of RNA isolation from human ocular tissues and analysis of prostanoid receptor mRNA expression using RT-PCR.","year":2002,"lang":"en","type":"article","venue":"PubMed","topic":"Glaucoma and retinal disorders","field":"Medicine","cited_by":23,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"RNA extraction; Prostanoid; RNA; Biology; Molecular biology; Complementary DNA; Messenger RNA; Receptor; Biochemistry; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002106169,0.0009814519,0.0006109067,0.0006467861,0.0004564112,0.0006278878,0.0005310982,0.0004100058,0.001783171],"category_scores_gemma":[0.001568431,0.0004120698,0.0006575878,0.0006108441,0.0005281445,0.0002958897,0.0003325342,0.0007305225,0.001318833],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002906288,"about_ca_system_score_gemma":0.0007385531,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004052656,"about_ca_topic_score_gemma":0.001309559,"domain_scores_codex":[0.9986161,0.0003496005,0.0002192025,0.0003838885,0.0003220815,0.0001090746],"domain_scores_gemma":[0.9991633,0.0003104559,0.0001288375,0.0001079191,0.0002632577,0.00002626131],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005231663,0.00003623936,0.0002214779,0.0002115605,0.00000975452,0.00008069597,0.00004160387,0.000148881,0.9936072,0.00007733483,0.00009312334,0.005419837],"study_design_scores_gemma":[0.00004099354,0.0007180477,0.004684555,0.00008015077,0.0001017583,0.0005814692,0.00007911416,0.001563686,0.9801913,0.0001979098,0.0117303,0.00003057917],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3433591,0.01223512,0.621054,0.0004389233,0.0004771561,0.008074268,0.004706448,0.001476686,0.00817832],"genre_scores_gemma":[0.2087043,0.008225468,0.7561026,0.0004623815,0.0001913738,0.009224962,0.01081217,0.0003303547,0.005946392],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.002106169,"threshold_uncertainty_score":0.01113868,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02442454110723686,"score_gpt":0.2435460058533331,"score_spread":0.2191214647460963,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}