{"id":"W2012563361","doi":"10.1016/j.pep.2008.12.005","title":"Bioprocess development for production, purification, and structural characterization of recombinant hCD83ext as a potential therapeutic protein","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":12,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Waterloo","funders":"","keywords":"Bioprocess; Recombinant DNA; Downstream processing; Trimer; Chemistry; Chromatography; Dimer; Tetramer; Affinity chromatography; Biochemistry; Yield (engineering); Protein purification; Fusion protein; Biology; Enzyme; Materials science; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006013718,0.00042524,0.0003830742,0.0002470927,0.0003591897,0.0004574539,0.000278189,0.0003186718,0.001094656],"category_scores_gemma":[0.0006210429,0.0001822241,0.000415408,0.0004087513,0.0002682408,0.0002817723,0.0002163383,0.0006452685,0.0005197904],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003911984,"about_ca_system_score_gemma":0.0004433712,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009446321,"about_ca_topic_score_gemma":0.001347308,"domain_scores_codex":[0.9997162,0.0000382513,0.00004970474,0.00005196529,0.00009643385,0.00004745023],"domain_scores_gemma":[0.99971,0.00008111036,0.00003972064,0.00006300354,0.00006981363,0.00003633182],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003807006,0.00002675513,0.0001163411,0.00002225638,0.000003076163,0.0000328294,0.00002375995,0.00011349,0.997758,0.0000726065,0.00002954149,0.001763303],"study_design_scores_gemma":[0.000003679674,0.00003212208,0.000673826,0.000002216094,0.000006015362,0.00005569258,0.000005542424,0.0004213572,0.9978503,0.00002415369,0.0009223818,0.000002683748],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9044452,0.001142614,0.09075186,0.000202841,0.00005055066,0.0003892675,0.00108289,0.0002615651,0.001673243],"genre_scores_gemma":[0.8549481,0.001918729,0.1328612,0.0001539793,0.00002419653,0.0004399852,0.005080082,0.0001646519,0.004409045],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001094656,"threshold_uncertainty_score":0.00366205,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01274519185144596,"score_gpt":0.246732396020703,"score_spread":0.2339872041692571,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}