{"id":"W2012948029","doi":"10.1128/aem.01387-10","title":"Relative and Absolute Quantitative Real-Time PCR-Based Quantifications of <i>hcnC</i> and <i>phlD</i> Gene Transcripts in Natural Soil Spiked with <i>Pseudomonas</i> sp. Strain LBUM300","year":2010,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Moncton","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; Real-time polymerase chain reaction; RNA extraction; TaqMan; Pseudomonas; Gene expression; Gene; Strain (injury); Bacteria; RNA; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00172033,0.001513772,0.001133773,0.0009636511,0.0003928098,0.001122283,0.001133632,0.001212255,0.002432142],"category_scores_gemma":[0.001911568,0.0006560429,0.00112622,0.001524746,0.001336595,0.0009078078,0.0005922864,0.001775288,0.0009769296],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008455403,"about_ca_system_score_gemma":0.0007539985,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006272165,"about_ca_topic_score_gemma":0.001168914,"domain_scores_codex":[0.9954522,0.0007934668,0.0003678508,0.001925207,0.001071387,0.0003897657],"domain_scores_gemma":[0.9981282,0.0006433613,0.0003658895,0.000245827,0.0005151703,0.0001014808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001148642,0.00003204107,0.0002318906,0.0001624171,0.000009730591,0.0000137755,0.00005358092,0.0001584945,0.9974557,0.0001248644,0.00006882747,0.001573838],"study_design_scores_gemma":[0.00001954963,0.0003944717,0.003590351,0.00002507696,0.00004063104,0.0000992194,0.00008347322,0.002610457,0.9895908,0.0001821893,0.003318436,0.0000453843],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6035129,0.005050546,0.3565347,0.0005246118,0.0006905415,0.001404514,0.02102117,0.00266927,0.008591843],"genre_scores_gemma":[0.4552489,0.003509867,0.5010682,0.0007878643,0.0002176836,0.005995988,0.01974889,0.000805279,0.01261739],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002432142,"threshold_uncertainty_score":0.009098053,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003936637943750865,"score_gpt":0.1962079575791666,"score_spread":0.1922713196354157,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}