{"id":"W2012948029","doi":"10.1128/aem.01387-10","title":"Relative and Absolute Quantitative Real-Time PCR-Based Quantifications of <i>hcnC</i> and <i>phlD</i> Gene Transcripts in Natural Soil Spiked with <i>Pseudomonas</i> sp. Strain LBUM300","year":2010,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Molecular Biology Techniques and Applications","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Moncton","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Biology; Real-time polymerase chain reaction; RNA extraction; TaqMan; Pseudomonas; Gene expression; Gene; Strain (injury); Bacteria; RNA; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001306749,0.0002204347,0.0002479254,0.00004997716,0.00008948332,0.000007490237,0.00009684602,0.0002536204,0.000006807552],"category_scores_gemma":[0.000003401207,0.0001948366,0.00003279021,0.00005498372,0.00106104,0.000005724878,0.00006360682,0.0002026294,0.000001519517],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00000626555,"about_ca_system_score_gemma":0.00002098369,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003873456,"about_ca_topic_score_gemma":0.0001319634,"domain_scores_codex":[0.9989324,0.00004343715,0.0002472062,0.0005265168,0.00002928831,0.0002211089],"domain_scores_gemma":[0.9995482,0.00003102145,0.000116519,0.0002340826,0.000007521298,0.00006264121],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001291671,0.00009577345,0.001053959,0.00001057893,0.00003515253,0.00000148543,0.0000864851,0.000004069178,0.996587,0.001585136,0.00004035123,0.0003708734],"study_design_scores_gemma":[0.001415022,0.0004065131,0.0127633,0.00001022057,0.00004450133,0.00008241598,0.0001310988,0.00005632937,0.9828184,0.0002717895,0.001673054,0.000327412],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9975228,0.0003803998,0.0008352687,0.0001892519,0.00001502288,0.0004158677,0.0003915335,0.00001178993,0.0002380886],"genre_scores_gemma":[0.9875594,0.0005260937,0.01035655,0.0002180231,0.00001166255,0.00007797535,0.001179147,0.00002215022,0.00004892676],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01376862,"threshold_uncertainty_score":0.7945205,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003936637943750865,"score_gpt":0.1962079575791666,"score_spread":0.1922713196354157,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}