{"id":"W2013864739","doi":"10.1006/prep.2000.1291","title":"Expression and Purification of Mammalian Calreticulin in Pichia pastoris","year":2000,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; University of Alberta","funders":"","keywords":"Calreticulin; Pichia pastoris; Endoplasmic reticulum; Recombinant DNA; Chaperone (clinical); Biochemistry; Biology; Molecular biology; Affinity chromatography; Binding protein; Calcium-binding protein; Calsequestrin; Chemistry; Enzyme; Calcium; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001433597,0.0001340144,0.0001286315,0.00005279356,0.00006647981,0.00002093908,0.00008682532,0.0001500038,0.00009187455],"category_scores_gemma":[0.00005176501,0.0001162365,0.00002904328,0.00006888827,0.00008344674,0.00001382512,0.00003080208,0.00007564898,0.000003443979],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006028852,"about_ca_system_score_gemma":0.00002139189,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003826112,"about_ca_topic_score_gemma":0.00000520207,"domain_scores_codex":[0.9989605,0.0001110715,0.0002844682,0.00037147,0.0001307012,0.0001417657],"domain_scores_gemma":[0.9994511,0.000007677595,0.00009693568,0.0002931823,0.00004062569,0.0001105069],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000197598,0.00009526985,0.003461659,0.00007237725,0.000002046224,7.511977e-7,0.0001449479,0.00001957658,0.9787741,0.0001013186,0.0001744099,0.01695595],"study_design_scores_gemma":[0.0008211561,0.00009500168,0.02096246,0.0001548633,0.000007081507,0.00000317094,0.0001104353,0.0001889282,0.9687088,0.0001911353,0.008590073,0.0001669143],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9961681,0.002188872,0.0003444515,0.0001948638,0.00002211234,0.0004422483,0.00001738021,0.000009522661,0.0006124843],"genre_scores_gemma":[0.9963734,0.0008522931,0.001839684,0.00003019262,0.00005403537,0.0001529632,0.000111204,0.00001280497,0.0005734517],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0175008,"threshold_uncertainty_score":0.4739986,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007038584726515236,"score_gpt":0.2317656977747928,"score_spread":0.2247271130482775,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}