{"id":"W2013864739","doi":"10.1006/prep.2000.1291","title":"Expression and Purification of Mammalian Calreticulin in Pichia pastoris","year":2000,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Endoplasmic Reticulum Stress and Disease","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto; University of Alberta","funders":"","keywords":"Calreticulin; Pichia pastoris; Endoplasmic reticulum; Recombinant DNA; Chaperone (clinical); Biochemistry; Biology; Molecular biology; Affinity chromatography; Binding protein; Calcium-binding protein; Calsequestrin; Chemistry; Enzyme; Calcium; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006767623,0.00114755,0.001443803,0.000821326,0.0007380209,0.001063376,0.001044068,0.000589726,0.001132832],"category_scores_gemma":[0.0004681292,0.000671226,0.0006387761,0.001863935,0.0004107268,0.0005550631,0.0007168353,0.001694179,0.001750887],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001397258,"about_ca_system_score_gemma":0.0005468279,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004620033,"about_ca_topic_score_gemma":0.004628598,"domain_scores_codex":[0.999409,0.00008138933,0.00008851958,0.0001270498,0.0001842812,0.0001097301],"domain_scores_gemma":[0.999754,0.00004757735,0.00003413814,0.00006047978,0.00005426938,0.00004967373],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002424129,0.0001129265,0.0004151812,0.0002239946,0.00004141023,0.0001745843,0.0001301032,0.0005282486,0.9921407,0.0005907923,0.0006962742,0.004703378],"study_design_scores_gemma":[0.0001078904,0.0002012001,0.005914906,0.00005016737,0.00009508344,0.0003653559,0.00006563245,0.004374952,0.9533798,0.0002947002,0.03511114,0.00003925358],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8385849,0.008775295,0.1050531,0.001751077,0.0009008871,0.001565141,0.02760101,0.002514447,0.01325405],"genre_scores_gemma":[0.7127888,0.008888879,0.144571,0.000650474,0.0001983033,0.001265982,0.08080762,0.0008055202,0.05002336],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004620033,"threshold_uncertainty_score":0.0101378,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007038584726515236,"score_gpt":0.2317656977747928,"score_spread":0.2247271130482775,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}