{"id":"W2014205849","doi":"10.1016/j.ab.2012.01.004","title":"Development of a continuous assay and steady-state characterization of Escherichia coli threonine synthase","year":2012,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":false,"ca_institutions":"Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Escherichia coli; Threonine; Chemistry; Biochemistry; ATP synthase; Biology; Enzyme; Serine; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001088478,0.0007554319,0.0006996048,0.0006905289,0.0003047443,0.001223449,0.0009404966,0.0007581074,0.000633276],"category_scores_gemma":[0.001001161,0.0006413699,0.000415017,0.0006376462,0.0004692312,0.0007401006,0.0007078794,0.001503744,0.0008438632],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007489174,"about_ca_system_score_gemma":0.0009533686,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002066158,"about_ca_topic_score_gemma":0.002604653,"domain_scores_codex":[0.9990927,0.0001631009,0.0001035187,0.0002140594,0.0003350205,0.0000915847],"domain_scores_gemma":[0.9989255,0.0003652454,0.00009016056,0.0002231849,0.0002600943,0.0001357856],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000461791,0.0001001354,0.0002177652,0.00002359048,0.000005314911,0.000008687693,0.00001434275,0.0000955645,0.9969221,0.0001244038,0.00005643615,0.002385395],"study_design_scores_gemma":[0.00001257109,0.0001226207,0.00108995,0.000003591857,0.00001066239,0.00005497188,0.00001539116,0.002113994,0.9955623,0.0000756529,0.0009279018,0.0000104181],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7265733,0.001626083,0.2604896,0.001054951,0.0002416229,0.0008070365,0.004173133,0.002108551,0.00292578],"genre_scores_gemma":[0.8699917,0.001026769,0.1163871,0.0002524694,0.00006136714,0.000504735,0.006336024,0.000277414,0.005162391],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.002066158,"threshold_uncertainty_score":0.005756557,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007008967597925862,"score_gpt":0.2228356634054163,"score_spread":0.2158266958074904,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}