{"id":"W2014426503","doi":"10.1128/aem.01768-10","title":"Versatile Enzyme Expression and Characterization System for Aspergillus nidulans, with the Penicillium brevicompactum Polyketide Synthase Gene from the Mycophenolic Acid Gene Cluster as a Test Case","year":2011,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":104,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Fondation Chalmers; Danmarks Frie Forskningsfond","keywords":"Aspergillus nidulans; Polyketide synthase; Gene cluster; Gene; Biology; Aspergillus; Enzyme; Biochemistry; Penicillium; Gene expression; Mycophenolic acid; Genetics; Polyketide; Chemistry; Biosynthesis; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001071849,0.0002326586,0.000280326,0.00002056149,0.0002816267,0.00001774067,0.00008409171,0.0001270211,0.00009659814],"category_scores_gemma":[0.000006607442,0.0001117467,0.00003667633,0.0000293903,0.0002788731,0.00003483685,0.0001032065,0.0001274319,0.00001626968],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004412406,"about_ca_system_score_gemma":0.00001025039,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001498043,"about_ca_topic_score_gemma":0.00001295136,"domain_scores_codex":[0.9990893,0.00004537055,0.0001847719,0.0004059662,0.00003686354,0.000237716],"domain_scores_gemma":[0.9994026,0.000121089,0.0001186065,0.0002776895,0.000005152466,0.00007489323],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005727043,0.00006565043,0.0003934833,0.00003154557,0.00006170638,0.00003031701,0.0006661827,1.131432e-7,0.9966961,0.000006722624,0.0001279779,0.001347536],"study_design_scores_gemma":[0.001385501,0.0002648195,0.003065554,0.00004492869,0.0002344761,0.003232328,0.0009706568,0.00001206674,0.9862853,0.000002326085,0.00432195,0.00018012],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9963332,0.0009542795,0.00009849206,0.0006385433,0.00005751841,0.0009974401,0.000872054,0.00002058346,0.00002790483],"genre_scores_gemma":[0.9971716,0.0001877648,0.0003885566,0.001258948,0.0001853429,0.00002159275,0.0007066603,0.0000269173,0.00005262341],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01041079,"threshold_uncertainty_score":0.4556896,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007867417216640733,"score_gpt":0.1731116405703097,"score_spread":0.165244223353669,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}