{"id":"W2014426503","doi":"10.1128/aem.01768-10","title":"Versatile Enzyme Expression and Characterization System for Aspergillus nidulans, with the Penicillium brevicompactum Polyketide Synthase Gene from the Mycophenolic Acid Gene Cluster as a Test Case","year":2011,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Microbial Natural Products and Biosynthesis","field":"Medicine","cited_by":104,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Fondation Chalmers; Danmarks Frie Forskningsfond","keywords":"Aspergillus nidulans; Polyketide synthase; Gene cluster; Gene; Biology; Aspergillus; Enzyme; Biochemistry; Penicillium; Gene expression; Mycophenolic acid; Genetics; Polyketide; Chemistry; Biosynthesis; Mutant","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004190653,0.0006749752,0.0005073509,0.000622501,0.0002553361,0.0004489682,0.0005260931,0.0003284847,0.0009682412],"category_scores_gemma":[0.0002985921,0.0003108745,0.0005492053,0.0005192521,0.0001869529,0.000283622,0.0004274004,0.0009286573,0.001806015],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003943481,"about_ca_system_score_gemma":0.0004398001,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00137789,"about_ca_topic_score_gemma":0.001820059,"domain_scores_codex":[0.9994973,0.00007061588,0.0001096678,0.0001129588,0.0001645762,0.00004492365],"domain_scores_gemma":[0.9997886,0.00003326444,0.00004016753,0.00006266111,0.00003642783,0.00003891923],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002762586,0.00002042898,0.0001495724,0.00002925403,0.00000428458,0.00003793841,0.00001207634,0.00005557351,0.9977263,0.0001155527,0.00005842574,0.001763096],"study_design_scores_gemma":[0.00001334377,0.00008246817,0.002293387,0.00001179069,0.00002341497,0.0003381238,0.00002114448,0.001191137,0.9876694,0.00009781185,0.00824409,0.00001395244],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6301795,0.002180622,0.3466122,0.0005414848,0.0001605142,0.001354324,0.01278051,0.002122276,0.004068593],"genre_scores_gemma":[0.6188483,0.001825818,0.3335673,0.0001700258,0.00003234948,0.001385866,0.0339701,0.0005410846,0.009659025],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00137789,"threshold_uncertainty_score":0.003239095,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007867417216640733,"score_gpt":0.1731116405703097,"score_spread":0.165244223353669,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}