{"id":"W2015884905","doi":"10.1016/j.bbrc.2005.07.051","title":"SMR proteins SugE and EmrE bind ligand with similar affinity and stoichiometry","year":2005,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"ExxonMobil Research and Engineering Company","keywords":"Isothermal titration calorimetry; Dissociation constant; Chemistry; Membrane; Stoichiometry; Titration; GroEL; Membrane protein; Escherichia coli; Ligand (biochemistry); Enthalpy; Plasma protein binding; Binding site; Biophysics; Biochemistry; Biology; Inorganic chemistry; Physical chemistry; Receptor","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008412107,0.0006696548,0.001317543,0.0006047128,0.0005674003,0.001203873,0.0008536291,0.001347276,0.004897224],"category_scores_gemma":[0.001394847,0.0005467375,0.0007683105,0.0002971842,0.0005060756,0.0008083766,0.0007094334,0.00114567,0.003269884],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007449058,"about_ca_system_score_gemma":0.0001920088,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004962471,"about_ca_topic_score_gemma":0.0007901829,"domain_scores_codex":[0.9993026,0.0001107848,0.00005739179,0.0001239451,0.0002639187,0.0001413016],"domain_scores_gemma":[0.999286,0.0002080598,0.00007404026,0.0001512129,0.0001622041,0.0001184685],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001896022,0.00006788934,0.0005262711,0.00009798326,0.00003147117,0.0001188686,0.00003544464,0.0001141316,0.9942555,0.001286401,0.0003532624,0.002923029],"study_design_scores_gemma":[0.00006729678,0.0001785986,0.006538799,0.0000121536,0.00003001706,0.001290129,0.0001407087,0.002626136,0.9770303,0.000557973,0.0114936,0.00003423826],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9294813,0.002951237,0.04534131,0.0009023526,0.0002134798,0.0002108395,0.0008201633,0.00141311,0.01866615],"genre_scores_gemma":[0.9516026,0.0008303464,0.02765132,0.000777247,0.00008866688,0.0001921787,0.002080124,0.0002356735,0.01654179],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004897224,"threshold_uncertainty_score":0.01638287,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04056789723012456,"score_gpt":0.3409516567008944,"score_spread":0.3003837594707699,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}