{"id":"W2016584091","doi":"10.1038/nmeth884","title":"Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase","year":2006,"lang":"en","type":"article","venue":"Nature Methods","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":101,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Deutsche Forschungsgemeinschaft; Stem Cell Network","keywords":"Recombinase; Embryonic stem cell; Cre recombinase; Biology; Stem cell; Cell biology; Cre-Lox recombination; Transduction (biophysics); Mutagenesis; Homologous recombination; Gene targeting; Site-specific recombination; Genetics; Transgene; Molecular biology; Recombination; Mutation; Gene; Genetically modified mouse; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001692081,0.000292356,0.0002950939,0.000203373,0.0001309409,0.00006384386,0.0004157347,0.0009792204,0.00005582667],"category_scores_gemma":[0.00002840157,0.0002938393,0.0001378383,0.0003773613,0.00004796365,0.000008599832,0.0001724442,0.001010235,0.00002570214],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001352024,"about_ca_system_score_gemma":0.00006091667,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00006003271,"about_ca_topic_score_gemma":0.00005213921,"domain_scores_codex":[0.9968646,0.001091552,0.0003923451,0.0007891924,0.0003208052,0.0005415399],"domain_scores_gemma":[0.9988915,0.00008113139,0.0001475592,0.0006325417,0.0001409968,0.0001062088],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004195918,0.0002777427,0.0002025758,0.00004066921,0.000007604158,0.00001221501,0.00001778251,0.00002087773,0.9739189,0.00007639581,0.02010232,0.005281011],"study_design_scores_gemma":[0.001148926,0.000216387,0.0008897833,0.0000186522,0.000006447393,0.000003521332,0.00003096953,0.00006437823,0.9211213,0.0002365567,0.07595134,0.0003117689],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9864978,0.005258096,0.0009532763,0.0001991079,0.0004953601,0.0004937207,0.00002680015,0.00002955056,0.00604628],"genre_scores_gemma":[0.9802793,0.0002740184,0.007042751,0.0001027676,0.0001579118,0.00004553295,0.0004367516,0.00006582829,0.0115952],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05584902,"threshold_uncertainty_score":0.9999514,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01669532389592018,"score_gpt":0.330080988804779,"score_spread":0.3133856649088589,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}