{"id":"W2016584091","doi":"10.1038/nmeth884","title":"Site-specific recombination in human embryonic stem cells induced by cell-permeant Cre recombinase","year":2006,"lang":"en","type":"article","venue":"Nature Methods","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":101,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Deutsche Forschungsgemeinschaft; Stem Cell Network","keywords":"Recombinase; Embryonic stem cell; Cre recombinase; Biology; Stem cell; Cell biology; Cre-Lox recombination; Transduction (biophysics); Mutagenesis; Homologous recombination; Gene targeting; Site-specific recombination; Genetics; Transgene; Molecular biology; Recombination; Mutation; Gene; Genetically modified mouse; Biochemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001004781,0.0006454847,0.0006271044,0.000596391,0.0003045625,0.0007820976,0.0009842911,0.0008638189,0.003487414],"category_scores_gemma":[0.0003498638,0.0007889438,0.000607005,0.0004199398,0.0005317889,0.0005886876,0.0005645523,0.001351694,0.002953297],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003910411,"about_ca_system_score_gemma":0.0004426575,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005725755,"about_ca_topic_score_gemma":0.0009109995,"domain_scores_codex":[0.9995155,0.00009421178,0.00006676768,0.0001106736,0.0001223957,0.00009051527],"domain_scores_gemma":[0.9997354,0.00006514315,0.00004857576,0.00007220311,0.00002775397,0.00005082914],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005953341,0.00001697915,0.00003428927,0.00003642187,0.000005900422,0.00004209378,0.00002238029,0.00003552937,0.9977982,0.0007849011,0.0001058193,0.001057913],"study_design_scores_gemma":[0.00004354664,0.00005879068,0.0004755957,0.000005639313,0.00001813898,0.0002437134,0.00001103305,0.0004256741,0.9918361,0.0001567769,0.006718786,0.000006277085],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7586204,0.004366789,0.2079567,0.0007701014,0.0005116074,0.001177134,0.004718982,0.00229577,0.01958254],"genre_scores_gemma":[0.8757289,0.002280783,0.07616213,0.0002611333,0.0000685333,0.0004691709,0.007667087,0.0006025414,0.03675982],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003487414,"threshold_uncertainty_score":0.01166654,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01669532389592018,"score_gpt":0.330080988804779,"score_spread":0.3133856649088589,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}