{"id":"W2016800171","doi":"10.1006/abio.2000.4761","title":"Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes","year":2000,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Proteoglycans and glycosaminoglycans research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":58,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Medical Research Council","keywords":"Toluidine; Staining; Chemistry; Glycoconjugate; Chromatography; Cellulose acetate; Chondrocyte; Glycosaminoglycan; Biochemistry; Cellulose; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001145811,0.0002233998,0.0002813128,0.00003180394,0.0001122044,0.00006647251,0.0001632429,0.0002324106,0.00002552127],"category_scores_gemma":[0.0001739997,0.0001728005,0.00007382465,0.0001287083,0.0003076354,0.00001389761,0.00007831112,0.0000965553,6.817045e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001260233,"about_ca_system_score_gemma":0.00004576474,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004702285,"about_ca_topic_score_gemma":0.00002838322,"domain_scores_codex":[0.9985701,0.000049264,0.0003741344,0.0005704185,0.0001942734,0.0002417485],"domain_scores_gemma":[0.9990555,0.0001125712,0.0001156753,0.0004223551,0.0001418929,0.0001520259],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000709877,0.0001032139,0.0002139935,0.0001079668,0.0001035973,4.080459e-7,0.00004474412,0.00001702829,0.9931947,0.00005941316,0.0001575541,0.005287533],"study_design_scores_gemma":[0.0007978473,0.0002438734,0.0004386954,0.00004198512,0.0001284368,0.000003335859,0.00005846336,0.006429608,0.9899471,0.00006171665,0.001649687,0.0001992679],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9621805,0.001473352,0.03426743,0.0006461729,0.000006813184,0.00105185,0.0003615253,0.000007308018,0.000005073541],"genre_scores_gemma":[0.9967439,0.0007158844,0.001571729,0.00005271493,0.00005410571,0.0002578935,0.0003916931,0.00002107197,0.0001909933],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03456343,"threshold_uncertainty_score":0.7046601,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007100698215867066,"score_gpt":0.2471894618510536,"score_spread":0.2400887636351865,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}