{"id":"W2016800171","doi":"10.1006/abio.2000.4761","title":"Differential Use of Alcian Blue and Toluidine Blue Dyes for the Quantification and Isolation of Anionic Glycoconjugates from Cell Cultures: Application to Proteoglycans and a High-Molecular-Weight Glycoprotein Synthesized by Articular Chondrocytes","year":2000,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Proteoglycans and glycosaminoglycans research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":58,"is_retracted":false,"has_abstract":false,"ca_institutions":"Queen's University","funders":"Medical Research Council","keywords":"Toluidine; Staining; Chemistry; Glycoconjugate; Chromatography; Cellulose acetate; Chondrocyte; Glycosaminoglycan; Biochemistry; Cellulose; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001009528,0.00117378,0.0007143826,0.0008911904,0.0007766689,0.0009746719,0.0008474095,0.0008726004,0.001327503],"category_scores_gemma":[0.001064437,0.0007484629,0.0005715447,0.001031332,0.001086429,0.001136076,0.001215545,0.001673906,0.0006929121],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005153983,"about_ca_system_score_gemma":0.001036486,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001295942,"about_ca_topic_score_gemma":0.003717152,"domain_scores_codex":[0.9990873,0.0001362434,0.0001010051,0.0002243237,0.0002774578,0.0001736431],"domain_scores_gemma":[0.9985164,0.0004663086,0.0002061776,0.0002799601,0.0003365027,0.0001946646],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006260323,0.00001252892,0.00008483119,0.00005302427,0.00000462136,0.00002585498,0.00004107676,0.00001031829,0.997635,0.0001898707,0.00001428026,0.001865996],"study_design_scores_gemma":[0.00001441157,0.0001352335,0.002007542,0.00001235139,0.00004046325,0.0004321255,0.00004057589,0.000573388,0.9939671,0.0001955984,0.002566751,0.00001454132],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6646928,0.009535578,0.3178424,0.0004540868,0.0003781934,0.0002005072,0.0003067163,0.0007415798,0.005848099],"genre_scores_gemma":[0.6191396,0.01109415,0.355619,0.000345088,0.0001524163,0.0006535935,0.001364618,0.0006575296,0.01097401],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001327503,"threshold_uncertainty_score":0.005338907,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007100698215867066,"score_gpt":0.2471894618510536,"score_spread":0.2400887636351865,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}