{"id":"W2016940132","doi":"10.1021/bi6026105","title":"Recovery of Oligomers and Cooperativity When Monomers of the M<sub>2</sub>Muscarinic Cholinergic Receptor Are Reconstituted into Phospholipid Vesicles","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Cooperativity; Tetramer; Chemistry; Digitonin; Muscarinic acetylcholine receptor; Vesicle; Receptor; Biophysics; Agonist; Oxotremorine; Cooperative binding; Monomer; Muscarinic acetylcholine receptor M2; Phospholipid; Biochemistry; Binding site; Stereochemistry; Membrane; Biology; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003841631,0.000529269,0.0004897906,0.0002568093,0.0002130151,0.0005778989,0.0005522675,0.0003449273,0.001128847],"category_scores_gemma":[0.0004341895,0.0004069748,0.0005009403,0.0001600373,0.0003031274,0.0003393634,0.000348102,0.0009501319,0.0009200826],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005548936,"about_ca_system_score_gemma":0.0001907948,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009565527,"about_ca_topic_score_gemma":0.0009991893,"domain_scores_codex":[0.9995452,0.00005971282,0.00004116782,0.0001141327,0.0001373067,0.0001026386],"domain_scores_gemma":[0.9994829,0.000133582,0.00009019297,0.0001141788,0.00008846958,0.00009062044],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000093765,0.00002797791,0.0003077833,0.0000151034,0.00001503827,0.00004546324,0.00004984283,0.0000745058,0.9987432,0.00006236619,0.00002260639,0.0005422299],"study_design_scores_gemma":[0.00001840762,0.0001587864,0.007223353,0.000007150144,0.00002978505,0.0002677242,0.00006148026,0.002517671,0.988793,0.00006821151,0.0008417465,0.00001270096],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9950601,0.0004632265,0.002774219,0.00006022396,0.00002439295,0.00003304876,0.0002081903,0.0001451815,0.001231407],"genre_scores_gemma":[0.9936295,0.0003580193,0.002379735,0.00006291935,0.00001057596,0.00007334022,0.0008120926,0.00007871432,0.002595173],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001128847,"threshold_uncertainty_score":0.004025996,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006262227567756163,"score_gpt":0.2065046399666575,"score_spread":0.2002424123989013,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}