{"id":"W2016940132","doi":"10.1021/bi6026105","title":"Recovery of Oligomers and Cooperativity When Monomers of the M<sub>2</sub>Muscarinic Cholinergic Receptor Are Reconstituted into Phospholipid Vesicles","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Cooperativity; Tetramer; Chemistry; Digitonin; Muscarinic acetylcholine receptor; Vesicle; Receptor; Biophysics; Agonist; Oxotremorine; Cooperative binding; Monomer; Muscarinic acetylcholine receptor M2; Phospholipid; Biochemistry; Binding site; Stereochemistry; Membrane; Biology; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000393532,0.0002044683,0.0002651764,0.00003076228,0.00008241587,0.00001262796,0.0002350466,0.0002694502,0.00002499762],"category_scores_gemma":[0.0001604574,0.0001733151,0.0001490744,0.0001320561,0.0002816432,0.000007417854,0.0001572706,0.0001293871,0.000001036189],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002747568,"about_ca_system_score_gemma":0.0001233541,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003154778,"about_ca_topic_score_gemma":0.00001467328,"domain_scores_codex":[0.9988146,0.00003456089,0.0004020817,0.0003784965,0.0001429802,0.0002272896],"domain_scores_gemma":[0.9989932,0.0000268223,0.0003272837,0.0004203456,0.0001305676,0.0001018278],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001355454,0.00005072249,0.00137522,0.00010207,0.00007281754,4.706428e-7,0.00006733659,0.00003392253,0.9926224,0.000009794856,0.0004474341,0.005082228],"study_design_scores_gemma":[0.0003984742,0.0000686664,0.0007209881,0.00008916513,0.00002194568,0.000006341734,0.0003039318,0.00001212052,0.9975688,0.00003851709,0.0005748791,0.000196121],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972875,0.001651308,0.0002782693,0.00004656622,0.0002819554,0.0001337665,0.00004174334,0.000008208114,0.0002706443],"genre_scores_gemma":[0.9983165,0.0005991494,0.0006997318,0.00006950218,0.0001587743,0.00000654246,0.00004260086,0.00002044201,0.00008679121],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004946412,"threshold_uncertainty_score":0.7067586,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006262227567756163,"score_gpt":0.2065046399666575,"score_spread":0.2002424123989013,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}