{"id":"W2016979788","doi":"10.1002/mrd.22014","title":"Non‐viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation","year":2011,"lang":"en","type":"article","venue":"Molecular Reproduction and Development","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Center for Research Resources; National Institutes of Health","keywords":"Nucleofection; Biology; Transfection; Germline; Transgene; Transplantation; Stem cell; Andrology; Transgenesis; Reproductive technology; Germ cell; Molecular biology; Immunology; Embryo; Cell biology; Genetics; Cell culture; Gene; Internal medicine; Cryopreservation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003840491,0.0002610982,0.000290121,0.0002377624,0.0001183657,0.0003159013,0.0003351507,0.000274155,0.001378869],"category_scores_gemma":[0.0002278633,0.0001717529,0.0002424209,0.0001036269,0.0003710165,0.0001722825,0.0002556233,0.0003634175,0.0007153858],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002601083,"about_ca_system_score_gemma":0.0002267848,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00070999,"about_ca_topic_score_gemma":0.001292515,"domain_scores_codex":[0.9997169,0.00003312461,0.00002422588,0.0000698578,0.0001115541,0.00004426078],"domain_scores_gemma":[0.9997503,0.00007535255,0.00006665057,0.00003063143,0.00003413229,0.00004303355],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001143483,0.000005687953,0.0001049055,0.00001502322,0.000002103315,0.00001971359,0.00001114975,0.00001407112,0.9992962,0.00006446472,0.00001435204,0.0004407888],"study_design_scores_gemma":[0.0000149155,0.0002161988,0.004826241,0.000008191435,0.00002070354,0.0003568473,0.00002832957,0.000586377,0.9905236,0.00004172011,0.003372607,0.000004304723],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9663016,0.001980395,0.02748019,0.0001068469,0.00007252366,0.0001179386,0.0004978291,0.000262412,0.003180212],"genre_scores_gemma":[0.9688867,0.00122995,0.01946122,0.00008648822,0.00002943332,0.00012351,0.001606877,0.0001951241,0.008380868],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001378869,"threshold_uncertainty_score":0.004612744,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008455026602905404,"score_gpt":0.1985533687181442,"score_spread":0.1900983421152388,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}