{"id":"W2016979788","doi":"10.1002/mrd.22014","title":"Non‐viral transfection of goat germline stem cells by nucleofection results in production of transgenic sperm after germ cell transplantation","year":2011,"lang":"en","type":"article","venue":"Molecular Reproduction and Development","topic":"Animal Genetics and Reproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Eunice Kennedy Shriver National Institute of Child Health and Human Development; National Center for Research Resources; National Institutes of Health","keywords":"Nucleofection; Biology; Transfection; Germline; Transgene; Transplantation; Stem cell; Andrology; Transgenesis; Reproductive technology; Germ cell; Molecular biology; Immunology; Embryo; Cell biology; Genetics; Cell culture; Gene; Internal medicine; Cryopreservation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004943042,0.0001977088,0.0002052662,0.0001420712,0.00004463259,0.000005844795,0.00005503715,0.0001559426,0.000007216336],"category_scores_gemma":[0.000005764132,0.0001994404,0.0000712993,0.0001752043,0.00008459167,0.00001606515,0.00001404783,0.00009537108,0.00000181157],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002381437,"about_ca_system_score_gemma":0.00005674392,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005218523,"about_ca_topic_score_gemma":0.00002909506,"domain_scores_codex":[0.9981554,0.00007132014,0.0006236446,0.0007911128,0.0001843068,0.00017419],"domain_scores_gemma":[0.9993156,0.000001471961,0.0001944977,0.0003127362,0.0001276044,0.00004812204],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001492707,0.0003251995,0.0010561,0.0002120751,0.00003174334,0.000001811909,0.001790992,0.0001499466,0.9840443,0.000002291883,0.00004233534,0.01085053],"study_design_scores_gemma":[0.000831242,0.0004272148,0.02240746,0.00003120716,0.00003265655,0.00002139955,0.0001393389,0.000007183545,0.9742417,0.000007153973,0.001646745,0.0002067331],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9937366,0.0006646159,0.004436625,0.00003855516,0.000426353,0.0004950749,0.00000870148,0.00001145957,0.0001819844],"genre_scores_gemma":[0.9971145,0.001106396,0.001083736,0.000009889201,0.00006430619,0.00003845492,0.00007450181,0.00002651748,0.0004816488],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02135136,"threshold_uncertainty_score":0.8132945,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008455026602905404,"score_gpt":0.1985533687181442,"score_spread":0.1900983421152388,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}