{"id":"W2016984068","doi":"10.1006/abio.2000.4562","title":"Quantitation of Inhibition of DNA Methylation of the Retinoic Acid Receptor β Gene by 5-Aza-2′-deoxycytidine in Tumor Cells Using a Single-Nucleotide Primer Extension Assay","year":2000,"lang":"en","type":"article","venue":"Analytical Biochemistry","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal; Centre Hospitalier Universitaire Sainte-Justine","funders":"","keywords":"DNA methylation; Molecular biology; Methylation; CpG site; Bisulfite sequencing; Gene; Primer (cosmetics); DNA; Biology; Primer extension; Methylated DNA immunoprecipitation; Illumina Methylation Assay; genomic DNA; Cancer research; Gene expression; Chemistry; Nucleotide; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.002277504,0.003053842,0.001822729,0.002547815,0.0009180194,0.0007867166,0.001554557,0.001214694,0.002767939],"category_scores_gemma":[0.001407793,0.002034088,0.001824739,0.001698131,0.001254872,0.0007275046,0.0008407051,0.004733852,0.001952922],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001229428,"about_ca_system_score_gemma":0.001540052,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003008528,"about_ca_topic_score_gemma":0.006129865,"domain_scores_codex":[0.9972948,0.0008535216,0.0002349171,0.0007884373,0.0005276866,0.0003005856],"domain_scores_gemma":[0.9973731,0.001149351,0.0002758135,0.0004490385,0.0004932862,0.000259393],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001681638,0.00006572172,0.0002841159,0.0000757203,0.00002900767,0.00002165754,0.0001058896,0.000183992,0.9970081,0.0002402243,0.00004952951,0.00176791],"study_design_scores_gemma":[0.00003272657,0.0003711474,0.002089459,0.00001401336,0.0000565099,0.00004973719,0.00003050743,0.00303686,0.9929873,0.0001213159,0.001192974,0.00001760352],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4469594,0.005269058,0.5318813,0.0003572673,0.0004472446,0.001261903,0.0032773,0.003092295,0.007454279],"genre_scores_gemma":[0.418745,0.003954704,0.546207,0.0003513528,0.0001209721,0.00313649,0.009551533,0.0006204505,0.01731252],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003053842,"threshold_uncertainty_score":0.01204479,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01573064454514257,"score_gpt":0.2576228331358011,"score_spread":0.2418921885906586,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}