{"id":"W2018152197","doi":"10.1016/j.pep.2007.07.010","title":"High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies","year":2007,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Berberine and alkaloids research","field":"Medicine","cited_by":30,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Deutsche Forschungsgemeinschaft","keywords":"Enzyme; Chemistry; Escherichia coli; Electrospray ionization; Biochemistry; Allergen; Nuclear magnetic resonance spectroscopy; Enzyme assay; Protein secondary structure; ATP synthase; Recombinant DNA; Two-dimensional nuclear magnetic resonance spectroscopy; Benzylisoquinoline; Mass spectrometry; Stereochemistry; Biosynthesis; Biology; Chromatography; Gene; Allergy","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006665126,0.0002338077,0.0004691575,0.0001912994,0.0001950778,0.0000295454,0.0001250322,0.0002466167,0.0000699555],"category_scores_gemma":[0.0007044108,0.0001797749,0.00006283242,0.0001759879,0.0001779758,0.0001651571,0.0001017101,0.0002468386,0.000003958196],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003083434,"about_ca_system_score_gemma":0.00004718475,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001307703,"about_ca_topic_score_gemma":0.00001754947,"domain_scores_codex":[0.9980583,0.00008600357,0.0006049498,0.0005525732,0.0003727124,0.0003255195],"domain_scores_gemma":[0.998299,0.0003952559,0.0002834899,0.0004761221,0.0003418287,0.0002043029],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001117961,0.0002529718,0.001634098,0.0004496606,0.00004333721,0.000004980834,0.0004572586,5.334783e-7,0.9737485,0.0005737753,0.0007392925,0.02097762],"study_design_scores_gemma":[0.001883717,0.0004874169,0.01154944,0.0007729932,0.00005055323,0.000007825133,0.0003825848,0.00007949904,0.9797024,0.001264319,0.003636197,0.0001831176],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9834036,0.004838238,0.006892467,0.002262905,0.00006484574,0.002273178,0.00005753206,0.00007092632,0.0001362917],"genre_scores_gemma":[0.9758485,0.0009724128,0.02088329,0.00008919062,0.0002508152,0.0005139076,0.0001768003,0.0000298575,0.001235214],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02079451,"threshold_uncertainty_score":0.7331006,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04036441475721649,"score_gpt":0.3230007188441224,"score_spread":0.2826363040869059,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}