{"id":"W2018152197","doi":"10.1016/j.pep.2007.07.010","title":"High-yield expression and purification of isotopically labeled norcoclaurine synthase, a Bet v 1-homologous enzyme, from Thalictrum flavum for NMR studies","year":2007,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Berberine and alkaloids research","field":"Medicine","cited_by":30,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Deutsche Forschungsgemeinschaft","keywords":"Enzyme; Chemistry; Escherichia coli; Electrospray ionization; Biochemistry; Allergen; Nuclear magnetic resonance spectroscopy; Enzyme assay; Protein secondary structure; ATP synthase; Recombinant DNA; Two-dimensional nuclear magnetic resonance spectroscopy; Benzylisoquinoline; Mass spectrometry; Stereochemistry; Biosynthesis; Biology; Chromatography; Gene; Allergy","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003404735,0.0004631741,0.0004904082,0.000199147,0.0003505841,0.0003755805,0.0004028549,0.0003370754,0.0005301709],"category_scores_gemma":[0.0002849007,0.0002126735,0.0002622413,0.0004911497,0.0002388724,0.0002701047,0.0002493568,0.0006954001,0.0004338337],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007540811,"about_ca_system_score_gemma":0.0003995464,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002366512,"about_ca_topic_score_gemma":0.004455965,"domain_scores_codex":[0.9998413,0.00001810103,0.00001222675,0.0000354381,0.00005603179,0.00003675405],"domain_scores_gemma":[0.9998618,0.00003190585,0.00002334362,0.00002123789,0.00002838252,0.00003325006],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003481582,0.00001745462,0.00008142702,0.00003863991,0.000003003372,0.00003295937,0.00002738679,0.00008672657,0.9986254,0.0001195229,0.00007027894,0.0008625843],"study_design_scores_gemma":[0.00002406986,0.00007603893,0.002859931,0.000009866098,0.00002207983,0.0001983285,0.00003931425,0.001579122,0.9882962,0.0001177769,0.006766966,0.00001039457],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.959042,0.001843358,0.03029913,0.0005658719,0.0001214345,0.0002265269,0.004310342,0.0002443524,0.003346989],"genre_scores_gemma":[0.9370215,0.001871959,0.03741214,0.0001208301,0.00004605651,0.0001719602,0.01462449,0.00016531,0.008565681],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002366512,"threshold_uncertainty_score":0.005471289,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04036441475721649,"score_gpt":0.3230007188441224,"score_spread":0.2826363040869059,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}