{"id":"W2018461823","doi":"10.3791/52427","title":"Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cystic Fibrosis Research Advances","field":"Medicine","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; University of Toronto; Hospital for Sick Children","funders":"National Cancer Institute","keywords":"Cystic fibrosis transmembrane conductance regulator; Chloride channel; Potentiator; Phosphorylation; Mutant; Population; Cystic fibrosis; Biochemistry; Chemistry; Wild type; Cell biology; Membrane protein; Biology; ATP-binding cassette transporter; Transporter; Membrane; Genetics; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007155437,0.0001141712,0.000362789,0.0001741951,0.0000433816,0.00001808613,0.00004197877,0.00005457862,0.00002886451],"category_scores_gemma":[0.0006150185,0.00008867685,0.00004643099,0.0001058493,0.0001668055,0.0002976908,0.00004151946,0.000145567,0.000001721863],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001293547,"about_ca_system_score_gemma":0.0002993587,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002148685,"about_ca_topic_score_gemma":0.000001009183,"domain_scores_codex":[0.9984471,0.0001024887,0.0003575734,0.0001322951,0.0008048294,0.0001556997],"domain_scores_gemma":[0.9987979,0.00002868848,0.0003281418,0.00009150329,0.0004517818,0.000301999],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.006218875,0.0004033081,0.0018166,0.00007076364,0.0001548412,0.00001792236,0.0003011465,0.000002796118,0.9886396,0.00002632568,0.0002109248,0.002136893],"study_design_scores_gemma":[0.008669652,0.001914106,0.006936334,0.0004603111,0.00004846157,0.0003083358,0.0003117557,0.00009752025,0.9804403,0.0002113984,0.0005114744,0.00009035303],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955441,0.002147009,0.001315301,0.0001730257,0.0001804952,0.0003643657,0.000003067298,0.00000623128,0.0002663941],"genre_scores_gemma":[0.9959577,0.00004301478,0.003788678,0.00002170459,0.00009062276,0.000009677348,0.000001665093,0.000009365716,0.00007758921],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008199305,"threshold_uncertainty_score":0.3616137,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.14641933725393,"score_gpt":0.4471133776798801,"score_spread":0.3006940404259502,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}