{"id":"W2018461823","doi":"10.3791/52427","title":"Functional Reconstitution and Channel Activity Measurements of Purified Wildtype and Mutant CFTR Protein","year":2015,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Cystic Fibrosis Research Advances","field":"Medicine","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"SickKids Foundation; University of Toronto; Hospital for Sick Children","funders":"National Cancer Institute","keywords":"Cystic fibrosis transmembrane conductance regulator; Chloride channel; Potentiator; Phosphorylation; Mutant; Population; Cystic fibrosis; Biochemistry; Chemistry; Wild type; Cell biology; Membrane protein; Biology; ATP-binding cassette transporter; Transporter; Membrane; Genetics; Gene; Medicine","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001283786,0.000705287,0.0006612079,0.0004161903,0.0004260598,0.0004445787,0.0008108686,0.0008836809,0.00135271],"category_scores_gemma":[0.0006113965,0.0002755661,0.0005347495,0.0005800146,0.0005391706,0.0008038898,0.0004588053,0.001413567,0.000650828],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006068355,"about_ca_system_score_gemma":0.0003434488,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008286471,"about_ca_topic_score_gemma":0.0005679108,"domain_scores_codex":[0.9988895,0.0002440721,0.000185576,0.0002289616,0.0002938426,0.0001580146],"domain_scores_gemma":[0.9994656,0.0001480749,0.00006922983,0.0001202778,0.0001401359,0.00005672602],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005177898,0.00004668901,0.00005291355,0.00003623934,0.000004941197,0.0000186893,0.0000360611,0.00009095507,0.9988052,0.0001841922,0.0000538313,0.000618605],"study_design_scores_gemma":[0.00001138339,0.0001520277,0.0006009021,0.00000461054,0.0000101053,0.00006557996,0.00002884301,0.001316917,0.9962107,0.00007539452,0.001515688,0.000007850716],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.9270777,0.002202465,0.06258155,0.0003403089,0.0002121588,0.0004015913,0.004156411,0.0006625748,0.002365141],"genre_scores_gemma":[0.9225584,0.002612149,0.06254087,0.000139523,0.00004357989,0.0008415612,0.006299652,0.0003628084,0.004601445],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.00135271,"threshold_uncertainty_score":0.006789386,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.14641933725393,"score_gpt":0.4471133776798801,"score_spread":0.3006940404259502,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}