{"id":"W2018503930","doi":"10.1006/bbrc.2000.3063","title":"Purification of Dual-Tagged Intact Recombinant Proteins","year":2000,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":9,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Medical Research Council Canada","keywords":"FLAG-tag; Fusion protein; Affinity chromatography; Recombinant DNA; BamHI; Tandem affinity purification; Protein purification; Escherichia coli; HindIII; Biochemistry; Protein tag; Myc-tag; Agarose; Target protein; Chemistry; Molecular biology; Biology; Plasmid; DNA; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005490967,0.0007732482,0.0009136704,0.0005576807,0.0004263556,0.001181388,0.000888418,0.0005713552,0.002765887],"category_scores_gemma":[0.0004372656,0.0006425306,0.0007123805,0.0009450936,0.0003331161,0.00066451,0.0008072201,0.003216694,0.003854506],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001037903,"about_ca_system_score_gemma":0.0006547837,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001148573,"about_ca_topic_score_gemma":0.001349421,"domain_scores_codex":[0.9996175,0.00002186362,0.00004461685,0.0001048337,0.0001137157,0.00009759435],"domain_scores_gemma":[0.9996203,0.0000412072,0.00003362099,0.00007384639,0.0001005254,0.0001304754],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002311956,0.00009511912,0.0002750062,0.0001406568,0.0000245627,0.00008840772,0.00005602088,0.0001272905,0.9936243,0.0007842633,0.0009840857,0.003569082],"study_design_scores_gemma":[0.0000971219,0.0001269865,0.00395568,0.00002425775,0.00005220993,0.000602005,0.00006381107,0.002391424,0.944851,0.0005441572,0.04725649,0.00003493738],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8001049,0.009542178,0.1439663,0.001751314,0.001647299,0.0005924514,0.03109441,0.002152962,0.009148295],"genre_scores_gemma":[0.7022994,0.007884147,0.1363835,0.0009448654,0.0002576581,0.0005388745,0.08878369,0.0008224304,0.06208534],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002765887,"threshold_uncertainty_score":0.009252846,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05698948116938021,"score_gpt":0.3649488669049383,"score_spread":0.3079593857355581,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}