{"id":"W2019365078","doi":"10.1038/nprot.2012.038","title":"A simple and rapid nonviral approach to efficiently transfect primary tissue–derived cells using polyethylenimine","year":2012,"lang":"en","type":"article","venue":"Nature Protocols","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":111,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Polyethylenimine; Transfection; Stromal cell; Cell culture; Chemistry; Cationic polymerization; Molecular biology; Cell biology; Biophysics; Biology; Genetics; Cancer research","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002138693,0.0002201226,0.0002075591,0.00005971895,0.00008285289,0.00003866494,0.0001879943,0.0003855709,0.00001284642],"category_scores_gemma":[0.00001900451,0.0001928047,0.0000698429,0.0001092949,0.00004797646,0.00001107282,0.0001062079,0.0002427216,0.000008479995],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001760313,"about_ca_system_score_gemma":0.00004333146,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000968877,"about_ca_topic_score_gemma":0.000001550152,"domain_scores_codex":[0.9988123,0.00006975389,0.0001795843,0.000372723,0.0001543679,0.0004112795],"domain_scores_gemma":[0.9994195,0.00000816488,0.00004503307,0.0002928312,0.0000573883,0.0001770698],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002617417,0.0001639259,0.0004564584,0.00008134521,0.00002677616,7.904684e-7,0.0001397858,0.00003076453,0.9932817,0.000006881436,0.0008728416,0.004677006],"study_design_scores_gemma":[0.0006212985,0.0005562041,0.001608107,0.00002525613,0.00001788511,0.00001916778,0.00002797183,0.00004559454,0.9602385,0.000003466789,0.03657474,0.0002618844],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9649557,0.0009279506,0.005708037,0.00003294064,0.00008583267,0.0266169,0.00004283719,0.0000194741,0.001610301],"genre_scores_gemma":[0.9856387,0.000008735811,0.004884851,0.000948044,0.0004201424,0.00784428,0.00006890026,0.00003170371,0.0001545922],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03570189,"threshold_uncertainty_score":0.7862347,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01670460603210785,"score_gpt":0.3002913838120187,"score_spread":0.2835867777799109,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}