{"id":"W2019365078","doi":"10.1038/nprot.2012.038","title":"A simple and rapid nonviral approach to efficiently transfect primary tissue–derived cells using polyethylenimine","year":2012,"lang":"en","type":"article","venue":"Nature Protocols","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":111,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research","keywords":"Polyethylenimine; Transfection; Stromal cell; Cell culture; Chemistry; Cationic polymerization; Molecular biology; Cell biology; Biophysics; Biology; Genetics; Cancer research","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007735743,0.001046825,0.0005961731,0.0007683219,0.0006562331,0.0008209926,0.001056704,0.0008061946,0.00277807],"category_scores_gemma":[0.0003218588,0.0006737428,0.0008069816,0.0004153645,0.0004337855,0.0005916866,0.0006756635,0.002199757,0.002584826],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005849152,"about_ca_system_score_gemma":0.0007827074,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006685475,"about_ca_topic_score_gemma":0.002448434,"domain_scores_codex":[0.9991884,0.00008195749,0.00009538122,0.0001503766,0.0003961915,0.00008771649],"domain_scores_gemma":[0.9996803,0.00007369788,0.00005082227,0.0001068476,0.00004281566,0.00004559269],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001750346,0.00002267719,0.00003196916,0.0000424911,0.000005232353,0.00003992877,0.00001303872,0.00002066349,0.9963577,0.0002726441,0.0002811732,0.002895001],"study_design_scores_gemma":[0.00001404551,0.00008925578,0.0006812729,0.000007074244,0.00001518269,0.0004391702,0.000006977125,0.0003791832,0.9812288,0.000150487,0.016975,0.00001349894],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2264103,0.008148878,0.7262833,0.001183303,0.001135993,0.003388267,0.004384223,0.006538341,0.02252731],"genre_scores_gemma":[0.3998728,0.005789235,0.5225751,0.0007859948,0.000188356,0.002396155,0.01022392,0.001285324,0.05688305],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00277807,"threshold_uncertainty_score":0.009293616,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01670460603210785,"score_gpt":0.3002913838120187,"score_spread":0.2835867777799109,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}