{"id":"W2020400571","doi":"10.1074/jbc.m111.269563","title":"Energetics of Cyclic AMP Binding to HCN Channel C Terminus Reveal Negative Cooperativity","year":2011,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; University of California, San Francisco; Michael Smith Health Research BC","keywords":"Tetramer; Isothermal titration calorimetry; Allosteric regulation; Chemistry; Cooperative binding; Cooperativity; Protein subunit; Biophysics; Energetics; Binding site; Crystallography; Stereochemistry; Biochemistry; Biology; Receptor; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003015473,0.0003515524,0.0004284439,0.0001862112,0.0005086118,0.0003589049,0.0006801274,0.0003335922,0.002105778],"category_scores_gemma":[0.0006799025,0.0002167998,0.0002280901,0.0001310376,0.0005302123,0.0002991947,0.0002502264,0.0006107652,0.0002263159],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008086093,"about_ca_system_score_gemma":0.0003489682,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002259595,"about_ca_topic_score_gemma":0.002382573,"domain_scores_codex":[0.9997439,0.00002471803,0.000009274444,0.00005423675,0.0001033354,0.00006458192],"domain_scores_gemma":[0.9996319,0.0001871602,0.0000386134,0.00002796229,0.00005469334,0.00005971351],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006929763,0.0001920785,0.003761573,0.0001726548,0.00004946061,0.0004090536,0.000123141,0.0156113,0.9718226,0.002052063,0.0006063371,0.004506749],"study_design_scores_gemma":[0.0001252159,0.0004351863,0.02805538,0.00001455763,0.00006109743,0.0005354024,0.000174477,0.2775787,0.6890182,0.002854968,0.001069318,0.00007757935],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9963146,0.0002118283,0.00157819,0.00008770736,0.00001438229,0.00001132295,0.00004871595,0.0000354667,0.00169782],"genre_scores_gemma":[0.9990718,0.00007316624,0.0004045405,0.00002222056,0.000002982924,0.00001277088,0.00007112085,0.00001055887,0.000330746],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002259595,"threshold_uncertainty_score":0.007044494,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04915207043033525,"score_gpt":0.2682094957444993,"score_spread":0.2190574253141641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}