{"id":"W2020400571","doi":"10.1074/jbc.m111.269563","title":"Energetics of Cyclic AMP Binding to HCN Channel C Terminus Reveal Negative Cooperativity","year":2011,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Ion channel regulation and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Canadian Institutes of Health Research; University of California, San Francisco; Michael Smith Health Research BC","keywords":"Tetramer; Isothermal titration calorimetry; Allosteric regulation; Chemistry; Cooperative binding; Cooperativity; Protein subunit; Biophysics; Energetics; Binding site; Crystallography; Stereochemistry; Biochemistry; Biology; Receptor; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002554169,0.000133174,0.0002316415,0.00003185976,0.00004252854,0.000006473379,0.0001655286,0.000236312,0.00009954956],"category_scores_gemma":[0.0004235469,0.00009664699,0.0001413967,0.0001102303,0.0000934982,0.000005117843,0.0000758381,0.0001298679,0.000003874966],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001911608,"about_ca_system_score_gemma":0.00004174143,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000002231882,"about_ca_topic_score_gemma":4.4657e-7,"domain_scores_codex":[0.9991158,0.00004547314,0.000396686,0.0001673287,0.0001185137,0.0001561922],"domain_scores_gemma":[0.9991194,0.00001978948,0.0003173398,0.0001346506,0.0002679841,0.0001408584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003052647,0.0001190957,0.0008307503,0.00001368815,0.00003525677,0.000006698992,0.0001198483,0.00005080909,0.9973329,0.000003770455,0.0006335466,0.0005483113],"study_design_scores_gemma":[0.000285789,0.0003739503,0.002145847,0.00002647738,0.00001067913,0.0001088437,0.0001131762,0.00001380107,0.9941533,0.00005334417,0.00259081,0.0001239539],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958829,0.00006365378,0.002074971,0.00007582334,0.0002302967,0.000053981,0.00001597109,0.000004150137,0.001598202],"genre_scores_gemma":[0.9974266,0.00005815939,0.001101541,0.00011237,0.0005271444,0.000002711724,0.00002057673,0.000007703772,0.0007431876],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003179631,"threshold_uncertainty_score":0.394115,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04915207043033525,"score_gpt":0.2682094957444993,"score_spread":0.2190574253141641,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}