{"id":"W2020966616","doi":"10.1016/s0167-7012(03)00017-4","title":"A comparison of DNA extraction and purification methods to detect Escherichia coli O157:H7 in cattle manure","year":2003,"lang":"en","type":"article","venue":"Journal of Microbiological Methods","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":33,"is_retracted":false,"has_abstract":false,"ca_institutions":"Agriculture and Agri-Food Canada; Lakehead University","funders":"","keywords":"Manure; Chromatography; Escherichia coli; DNA extraction; Chloroform; Biology; Detection limit; Polymerase chain reaction; Extraction (chemistry); Food science; Microbiology; Chemistry; Biochemistry; Gene; Agronomy","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003286872,0.0008252949,0.0008670003,0.001896489,0.0005619277,0.0009194153,0.001127624,0.001296804,0.0008262084],"category_scores_gemma":[0.00452029,0.0007606039,0.0007968928,0.001208798,0.0006306589,0.0006920265,0.0004461741,0.0008611181,0.000519885],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004978871,"about_ca_system_score_gemma":0.0007367061,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0017834,"about_ca_topic_score_gemma":0.003751641,"domain_scores_codex":[0.9975131,0.0006651444,0.0002493196,0.0003093909,0.001077285,0.0001858408],"domain_scores_gemma":[0.9953668,0.002653725,0.0003664116,0.0002277226,0.001204119,0.0001811564],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009727234,0.0003072469,0.006593343,0.0004757558,0.00007653205,0.00006215428,0.0001606092,0.0001539642,0.9704055,0.0000596829,0.0001108439,0.02062165],"study_design_scores_gemma":[0.00009263925,0.003199452,0.04171379,0.00008180212,0.0003133781,0.0007433781,0.0003250846,0.001221602,0.9492373,0.0001203755,0.002907436,0.0000437098],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9517736,0.009778862,0.03511328,0.0004616496,0.0002408702,0.0003283895,0.0008602485,0.0001471012,0.001296023],"genre_scores_gemma":[0.8546715,0.01170564,0.1202281,0.0007213845,0.0001927325,0.0006279019,0.005188082,0.0001371453,0.006527544],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003286872,"threshold_uncertainty_score":0.0173828,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0625225840881217,"score_gpt":0.4540101300809437,"score_spread":0.391487545992822,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}