{"id":"W2021194106","doi":"10.1021/bi700837y","title":"Nucleotide Binding, ATP Hydrolysis, and Mutation of the Catalytic Carboxylates of Human P-Glycoprotein Cause Distinct Conformational Changes in the Transmembrane Segments","year":2007,"lang":"en","type":"article","venue":"Biochemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":24,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Nucleotide; ATP hydrolysis; Mutant; Cyclic nucleotide-binding domain; Chemistry; Stereochemistry; Biochemistry; Transmembrane domain; Binding site; Cysteine; Walker motifs; ATP-binding cassette transporter; Amino acid; Enzyme; ATPase; Transporter","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001572406,0.0004837891,0.0002454015,0.000140046,0.0001102158,0.0002387235,0.0002641509,0.0005794813,0.0007528572],"category_scores_gemma":[0.0003423874,0.0002844699,0.0003005046,0.0002071518,0.0003198598,0.0001966628,0.0002524441,0.0005276137,0.0001859509],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002916955,"about_ca_system_score_gemma":0.0001870272,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007648771,"about_ca_topic_score_gemma":0.000773735,"domain_scores_codex":[0.9996718,0.00006423582,0.00006446424,0.00006437147,0.00008611021,0.00004896698],"domain_scores_gemma":[0.9998424,0.0000361025,0.00005593474,0.00001936585,0.000006498306,0.00003966779],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004223328,0.0001218574,0.0003801122,0.00003023357,0.00001402647,0.0002294356,0.00002096926,0.0001915752,0.9973774,0.0001397571,0.000029357,0.00104303],"study_design_scores_gemma":[0.000108011,0.001070616,0.007348601,0.000006487096,0.00003010467,0.001854412,0.00002710368,0.001110456,0.9872856,0.00007286954,0.001072978,0.00001273878],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9987651,0.0003834213,0.0003419796,0.00004028504,0.00001291406,0.00002258043,0.0001121993,0.000008227162,0.0003134027],"genre_scores_gemma":[0.9980729,0.0002605055,0.0006836745,0.00005804066,0.000007267967,0.00004357708,0.0002995428,0.000007284621,0.0005671787],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007648771,"threshold_uncertainty_score":0.002518594,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009844810343996147,"score_gpt":0.2468000610436698,"score_spread":0.2369552506996737,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}