{"id":"W2021422325","doi":"10.1099/mic.0.030064-0","title":"Overcoming codon-usage bias in heterologous protein expression in Streptococcus gordonii","year":2009,"lang":"en","type":"article","venue":"Microbiology","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":true,"ca_institutions":"Izaak Walton Killam Health Centre; Dalhousie University","funders":"","keywords":"Streptococcus gordonii; Biology; Heterologous; Gene; Codon usage bias; Stop codon; Transfer RNA; Genetics; Heterologous expression; Microbiology; Molecular biology; Recombinant DNA; Streptococcus; Bacteria; RNA","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003847057,0.0002165625,0.0002754461,0.0001203474,0.0000393412,0.00001334533,0.0002617188,0.0004220577,0.00005699114],"category_scores_gemma":[0.00009108835,0.0001990849,0.00007414075,0.00009513371,0.00005047177,0.000004889535,0.00009749289,0.0001655807,0.00002198375],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003017649,"about_ca_system_score_gemma":0.0000352666,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002852804,"about_ca_topic_score_gemma":0.0001773164,"domain_scores_codex":[0.998341,0.0003272278,0.0003457753,0.0005074408,0.00003268377,0.0004459154],"domain_scores_gemma":[0.9994614,0.00001984609,0.0001023786,0.0003535957,0.00001713115,0.00004566367],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001686993,0.00009997519,0.002880735,0.000006968872,0.000004369801,0.00002707357,0.00004324023,0.00002386178,0.9908612,0.00002208663,0.0001340242,0.005727791],"study_design_scores_gemma":[0.0008463176,0.0005219021,0.0027112,0.00006383095,0.000001816205,0.00002458282,0.00002093167,0.000004928119,0.9842758,0.0002865431,0.01101848,0.0002236994],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976502,0.0006282792,0.0004439206,0.0001933715,0.00007975425,0.0006069971,0.00001614271,0.00001536503,0.0003659884],"genre_scores_gemma":[0.9976512,0.00003802341,0.001285187,0.0004963813,0.00006965869,0.00007544281,0.00009706379,0.00001613921,0.0002708862],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01088446,"threshold_uncertainty_score":0.8118445,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0157405797372589,"score_gpt":0.2459073734881711,"score_spread":0.2301667937509122,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}