{"id":"W2021554110","doi":"10.1128/aem.02900-06","title":"S-Layer-Mediated Display of the Immunoglobulin G-Binding Domain of Streptococcal Protein G on the Surface of<i>Caulobacter crescentus</i>: Development of an Immunoactive Reagent","year":2007,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Caulobacter crescentus; Immunoprecipitation; S-layer; Biology; Protein G; Fragment crystallizable region; Recombinant DNA; Molecular biology; Antibody; Immunoglobulin G; Protein A/G; Protein A; Biochemistry; Fucosylation; Binding protein; Chemistry; Glycoprotein; Glycan; Fusion protein; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001717961,0.0004802835,0.0001938191,0.0001698101,0.0001040889,0.0002644206,0.00036752,0.0003142716,0.0003742822],"category_scores_gemma":[0.00009750272,0.0001898888,0.0003019607,0.0001739905,0.0001660741,0.0001233421,0.0002472003,0.0004176861,0.0002822186],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003576493,"about_ca_system_score_gemma":0.0001737915,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001504755,"about_ca_topic_score_gemma":0.00149737,"domain_scores_codex":[0.9998882,0.00001916095,0.000008834905,0.00001905744,0.00003299174,0.00003174032],"domain_scores_gemma":[0.9999095,0.00001029066,0.00003012413,0.00001057445,0.00001457475,0.00002493533],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000009041053,0.000004585441,0.00002107037,0.000006165768,0.000001443684,0.00001184061,0.000003599535,0.00001900892,0.9997649,0.00001555662,0.000007238162,0.0001355918],"study_design_scores_gemma":[0.000003368546,0.0000534741,0.0004584472,0.000001378235,0.000003693727,0.0000458728,0.000005340808,0.0006563232,0.9983774,0.000004445159,0.0003878716,0.000002320482],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.992079,0.0003926274,0.006360068,0.00006822203,0.00001717899,0.00003465435,0.0001585114,0.00009632784,0.0007935858],"genre_scores_gemma":[0.9840733,0.0004763144,0.01288022,0.00006521257,0.000006544255,0.00002953456,0.0004948502,0.0000439686,0.001930004],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001504755,"threshold_uncertainty_score":0.002992034,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004194546056647535,"score_gpt":0.1825457059995686,"score_spread":0.178351159942921,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}