{"id":"W2021554110","doi":"10.1128/aem.02900-06","title":"S-Layer-Mediated Display of the Immunoglobulin G-Binding Domain of Streptococcal Protein G on the Surface of<i>Caulobacter crescentus</i>: Development of an Immunoactive Reagent","year":2007,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Biochemical and Structural Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Caulobacter crescentus; Immunoprecipitation; S-layer; Biology; Protein G; Fragment crystallizable region; Recombinant DNA; Molecular biology; Antibody; Immunoglobulin G; Protein A/G; Protein A; Biochemistry; Fucosylation; Binding protein; Chemistry; Glycoprotein; Glycan; Fusion protein; Receptor; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001615538,0.0001287894,0.0001730085,0.00001132909,0.00004621535,0.00000134068,0.0001574997,0.0001490045,0.00001567652],"category_scores_gemma":[0.000004961666,0.00007655817,0.00004846593,0.00003812024,0.0003809241,0.000002257295,0.0001485753,0.00008204615,5.596381e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001612041,"about_ca_system_score_gemma":0.00001424016,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005967492,"about_ca_topic_score_gemma":0.000002351668,"domain_scores_codex":[0.9992233,0.00004478354,0.0003545036,0.0001874945,0.00005047976,0.0001394721],"domain_scores_gemma":[0.9994788,0.00001970422,0.0002748828,0.0001969298,0.000007072527,0.00002267582],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000374351,0.0001073882,0.001760855,0.00001804113,0.00006239597,7.993459e-8,0.0002117402,0.00000196634,0.9967899,0.00005661195,0.000001746782,0.0006149461],"study_design_scores_gemma":[0.000349332,0.0001316584,0.04880232,0.0000193642,0.000008342909,0.000003184368,0.0003251054,5.733739e-7,0.9500191,0.00001232576,0.000251404,0.00007735266],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9992658,0.00005250489,0.00004248602,0.00001791048,0.00002519367,0.000380379,0.0001786487,0.000001170748,0.00003583977],"genre_scores_gemma":[0.9990821,0.00001637724,0.0003546218,0.00002378547,0.00001013093,0.000005275425,0.0004873187,0.000007489714,0.00001291446],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04704146,"threshold_uncertainty_score":0.3121952,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004194546056647535,"score_gpt":0.1825457059995686,"score_spread":0.178351159942921,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}