{"id":"W2021815097","doi":"10.1007/s002990050740","title":"Hygromycin resistance is an effective selectable marker for biolistic transformation of black spruce ( Picea mariana )","year":2000,"lang":"en","type":"article","venue":"Plant Cell Reports","topic":"Plant tissue culture and regeneration","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":false,"ca_institutions":"Natural Resources Canada; Canadian Forest Service","funders":"","keywords":"Biology; Selectable marker; Somatic embryogenesis; Hygromycin B; Southern blot; Transgene; Transformation (genetics); Molecular biology; Embryo; Gene; Beta-glucuronidase; Northern blot; Somatic cell; Genetics; Gene expression; Embryogenesis","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002640598,0.0005837342,0.0004430698,0.0005898277,0.0003713497,0.0004184272,0.0005872597,0.0004255731,0.001128707],"category_scores_gemma":[0.0001949261,0.000383495,0.0003437033,0.00032139,0.0002481709,0.0002414691,0.0005203362,0.001217401,0.00147181],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003100968,"about_ca_system_score_gemma":0.000187629,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006610082,"about_ca_topic_score_gemma":0.001713078,"domain_scores_codex":[0.9996784,0.00005359093,0.00003750209,0.00007668923,0.0001187809,0.00003497854],"domain_scores_gemma":[0.9997981,0.00004162971,0.00005758114,0.00003998675,0.00001874431,0.00004400498],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001575361,0.000009789861,0.00008114389,0.00002413853,0.000003123872,0.00003541585,0.00001298446,0.00001085203,0.9987203,0.00006445541,0.00005226119,0.000969881],"study_design_scores_gemma":[0.000008563208,0.00008935962,0.004435591,0.00001126356,0.00002732153,0.0003825043,0.00002218189,0.0003446464,0.9828135,0.00005733082,0.01179679,0.00001095901],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8722352,0.005342925,0.104116,0.0005649688,0.0003486213,0.0003025306,0.002764256,0.003083316,0.01124212],"genre_scores_gemma":[0.8984839,0.002458001,0.05764466,0.0002939535,0.00008942121,0.0003046502,0.008590244,0.0009576984,0.03117742],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001128707,"threshold_uncertainty_score":0.003775895,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006595512239059373,"score_gpt":0.2121445754102573,"score_spread":0.2055490631711979,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}