{"id":"W2022453152","doi":"10.1021/sb5003482","title":"Evolution of Synthetic Signaling Scaffolds by Recombination of Modular Protein Domains","year":2015,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; Ontario Ministry of Research and Innovation; Natural Sciences and Engineering Research Council of Canada; Ontario Ministry of Research, Innovation and Science; Canada Foundation for Innovation","keywords":"Synthetic biology; Scaffold; Scaffold protein; Modularity (biology); Modular design; Mating of yeast; Protein engineering; Computational biology; Biology; Protein domain; Signaling proteins; Signal transduction; Saccharomyces cerevisiae; Computer science; Cell biology; Genetics; Yeast; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006030602,0.0001473721,0.0002400193,0.00009540506,0.00003469295,0.000004670926,0.0002927528,0.0002946387,0.00001712895],"category_scores_gemma":[0.000297279,0.0001332191,0.00007996871,0.0001488478,0.0003182525,0.000003296868,0.0001484454,0.00006376777,0.000009645702],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003663419,"about_ca_system_score_gemma":0.0001450648,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007856042,"about_ca_topic_score_gemma":0.000005604074,"domain_scores_codex":[0.998579,0.00026765,0.0003483084,0.0003487284,0.0001775185,0.00027876],"domain_scores_gemma":[0.9989948,0.00002673244,0.0001699418,0.0003939855,0.0003139059,0.0001006017],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000106618,0.0001523299,0.0008611947,0.00003789905,0.00003931245,3.393748e-7,0.00003356188,0.00009909715,0.9954621,0.001349523,0.0001483611,0.001709627],"study_design_scores_gemma":[0.0004887115,0.001017876,0.0000847519,0.00003829112,0.00001640344,0.00000846587,0.00009011113,0.0002468389,0.9916903,0.004530062,0.001646725,0.0001414865],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9928688,0.001738445,0.004050528,0.0001811904,0.0001036417,0.0002959549,0.00005066117,0.000008098202,0.0007026715],"genre_scores_gemma":[0.9987484,0.0000496794,0.000730111,0.000009681854,0.00004557728,0.0000364384,0.00007905652,0.00002013143,0.0002809536],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005879561,"threshold_uncertainty_score":0.5432519,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01483939098091765,"score_gpt":0.2613701841238668,"score_spread":0.2465307931429492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}