{"id":"W2022453152","doi":"10.1021/sb5003482","title":"Evolution of Synthetic Signaling Scaffolds by Recombination of Modular Protein Domains","year":2015,"lang":"en","type":"article","venue":"ACS Synthetic Biology","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":11,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Conselho Nacional de Desenvolvimento Científico e Tecnológico; Ontario Ministry of Research and Innovation; Natural Sciences and Engineering Research Council of Canada; Ontario Ministry of Research, Innovation and Science; Canada Foundation for Innovation","keywords":"Synthetic biology; Scaffold; Scaffold protein; Modularity (biology); Modular design; Mating of yeast; Protein engineering; Computational biology; Biology; Protein domain; Signaling proteins; Signal transduction; Saccharomyces cerevisiae; Computer science; Cell biology; Genetics; Yeast; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004545904,0.0005494962,0.0003056198,0.0006333707,0.0001861855,0.0005139131,0.0004929345,0.0003733681,0.0004232073],"category_scores_gemma":[0.0007051105,0.0002988082,0.0003898483,0.0005267097,0.0003854377,0.0002602559,0.0006620709,0.0006574779,0.0002195877],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003893771,"about_ca_system_score_gemma":0.0002079947,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003005364,"about_ca_topic_score_gemma":0.0004209515,"domain_scores_codex":[0.9996699,0.00008129598,0.00003849838,0.00005319423,0.0000890535,0.00006814541],"domain_scores_gemma":[0.9996141,0.000122119,0.000143289,0.00004659217,0.00002574984,0.00004822011],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001885801,0.00009017332,0.0007639132,0.00004752144,0.00002817776,0.0001984599,0.00007331696,0.006584179,0.981756,0.002303566,0.00005081705,0.007915303],"study_design_scores_gemma":[0.00009735113,0.0006425825,0.003286388,0.00001779604,0.00007296076,0.0007877764,0.00008831007,0.03514602,0.9531901,0.0008782123,0.005756648,0.00003577293],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.977766,0.0001241384,0.02082243,0.00004717541,0.00001129414,0.00005185531,0.0001280641,0.0001219297,0.000926951],"genre_scores_gemma":[0.9760687,0.0002645447,0.02223696,0.00002352357,0.000006657983,0.00006369742,0.0004377436,0.00007946316,0.0008186399],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006333707,"threshold_uncertainty_score":0.002825201,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01483939098091765,"score_gpt":0.2613701841238668,"score_spread":0.2465307931429492,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}