{"id":"W2022564991","doi":"10.1021/bi020078n","title":"A Case for Reverse Protonation:  Identification of Glu160 as an Acid/Base Catalyst in <i>Thermoanaerobacterium saccharolyticum</i> β-Xylosidase and Detailed Kinetic Analysis of a Site-Directed Mutant","year":2002,"lang":"en","type":"article","venue":"Biochemistry","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Chemistry; Enzyme kinetics; Active site; Stereochemistry; Enzyme; Electrospray ionization; Biochemistry; Chromatography; Mass spectrometry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001734747,0.0001399323,0.0002201357,0.0001232978,0.00003296628,0.00001529865,0.00008043514,0.0001448953,0.00004975349],"category_scores_gemma":[0.0001984658,0.0001504378,0.0000832888,0.0004291902,0.00007159536,0.0000128355,0.00002822804,0.00004256127,7.029566e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001825397,"about_ca_system_score_gemma":0.00002464879,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000300768,"about_ca_topic_score_gemma":0.00005647439,"domain_scores_codex":[0.9988782,0.00004164913,0.0004530882,0.0004094117,0.0000972894,0.0001202941],"domain_scores_gemma":[0.9989637,0.000008359798,0.0003179506,0.0004030642,0.0002383456,0.00006860212],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001340427,0.0002196289,0.001757388,0.0001677605,0.0001121763,0.00001012609,0.0001553436,0.00001445873,0.9970971,0.000002924486,0.00002466435,0.0003043858],"study_design_scores_gemma":[0.0006692373,0.00009074719,0.001518179,0.00001492811,0.0002686155,0.00008731834,0.00009838027,0.004194383,0.992856,0.000002910616,0.00004934888,0.000149954],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9988652,0.0001783888,0.0001659734,0.00007098922,0.00002355599,0.000434,0.0002351459,0.00001288724,0.00001378657],"genre_scores_gemma":[0.9980364,0.00005124804,0.0002344956,0.00003735483,0.00004882407,0.0001252461,0.001276388,0.00001479092,0.0001751998],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004241103,"threshold_uncertainty_score":0.6134674,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01126031968171671,"score_gpt":0.2389776578235907,"score_spread":0.227717338141874,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}