{"id":"W2022631158","doi":"10.1016/j.pep.2003.09.007","title":"Deletion of the GPI pre-anchor sequence in human p97—a general approach for generating the soluble form of GPI-linked proteins","year":2004,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":23,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Recombinant DNA; Transfection; Chinese hamster ovary cell; Cell culture; Biology; Cleavage (geology); Cell; Biochemistry; Baby hamster kidney cell; Secretion; Cell biology; Gene; Molecular biology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003146098,0.0007000397,0.0004868971,0.0003039165,0.0003498197,0.0004908436,0.0007271614,0.0006578248,0.001191447],"category_scores_gemma":[0.0003104939,0.0004518694,0.0006515647,0.0004770622,0.0005604273,0.0003565147,0.0005125275,0.001844267,0.001416629],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003120096,"about_ca_system_score_gemma":0.0004841515,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007139413,"about_ca_topic_score_gemma":0.000874761,"domain_scores_codex":[0.9997823,0.00003698576,0.0000407293,0.00004511436,0.00007117265,0.00002374598],"domain_scores_gemma":[0.9997286,0.00008304322,0.0000648963,0.00006305809,0.00001750925,0.00004275221],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007411618,0.00002199715,0.00005854724,0.00003704055,0.000007032718,0.0001478964,0.00002037307,0.000145708,0.9976715,0.0003730238,0.00007679247,0.001366004],"study_design_scores_gemma":[0.00002272174,0.00005744931,0.0006591139,0.000005771374,0.00001524466,0.000512696,0.00001586313,0.0006922421,0.9936259,0.0001405205,0.004244098,0.000008363653],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.842375,0.001223094,0.1471062,0.0006351891,0.0002499075,0.000593833,0.004306552,0.0005984482,0.002911878],"genre_scores_gemma":[0.8744105,0.002125594,0.1005338,0.0002523842,0.00006179317,0.0004392794,0.01107575,0.0003574219,0.01074348],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001191447,"threshold_uncertainty_score":0.003985763,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01901811553973833,"score_gpt":0.2989297966052469,"score_spread":0.2799116810655086,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}