{"id":"W2022878025","doi":"10.4161/chan.19540","title":"Tryptophan scanning mutagenesis reveals distortions in the helical structure of the δM4 transmembrane domain of the<i>Torpedo californica</i>nicotinic acetylcholine receptor","year":2012,"lang":"en","type":"article","venue":"Channels","topic":"Nicotinic Acetylcholine Receptors Study","field":"Biochemistry, Genetics and Molecular Biology","cited_by":7,"is_retracted":false,"has_abstract":true,"ca_institutions":"Toronto Metropolitan University","funders":"National Institute of Neurological Disorders and Stroke; National Institute of General Medical Sciences; National Institutes of Health","keywords":"Torpedo; Transmembrane domain; Nicotinic acetylcholine receptor; Biophysics; Gating; Conformational change; Chemistry; Ion channel; Acetylcholine receptor; Protein structure; Protein subunit; Nicotinic agonist; Mutagenesis; Helix bundle; Crystallography; Receptor; Biology; Biochemistry; Mutation","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001234704,0.000208272,0.0001135492,0.00008967028,0.0001250924,0.0001511135,0.0002609981,0.0002189801,0.0003915906],"category_scores_gemma":[0.0001536015,0.0001064162,0.0002046089,0.00007868474,0.0001788526,0.00005433354,0.0001366928,0.0003262075,0.0001341622],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003213563,"about_ca_system_score_gemma":0.0001958018,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003042893,"about_ca_topic_score_gemma":0.002997292,"domain_scores_codex":[0.9999205,0.00001594088,0.000008479074,0.00001587514,0.00002340312,0.00001589929],"domain_scores_gemma":[0.9998823,0.00002551048,0.00005581409,0.000009845827,0.000009660687,0.00001684186],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002966737,0.00004289437,0.001734103,0.00004008548,0.00002111471,0.0003638001,0.00004246585,0.0006148076,0.9950782,0.00007779295,0.00005447011,0.001633535],"study_design_scores_gemma":[0.00007184554,0.0005704408,0.03736594,0.0000274003,0.00007411394,0.0008551236,0.0001982858,0.007468887,0.9513543,0.00008779945,0.001901921,0.00002391287],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9992174,0.00007449036,0.0003822334,0.00003038479,0.000004750637,0.000004387583,0.00005508033,0.00001572617,0.0002155707],"genre_scores_gemma":[0.9986846,0.00009499796,0.0007723213,0.00004033118,0.00000239352,0.000005176451,0.0001094878,0.000009901846,0.0002808872],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003042893,"threshold_uncertainty_score":0.006050408,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01171387190156843,"score_gpt":0.2563552230601395,"score_spread":0.2446413511585711,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}