{"id":"W2023267445","doi":"10.1021/ac034463+","title":"Use of Capillary Electrophoresis and Endogenous Fluorescent Substrate To Monitor Intracellular Activation of Protein Kinase A","year":2003,"lang":"en","type":"article","venue":"Analytical Chemistry","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":true,"ca_institutions":"York University","funders":"","keywords":"Capillary electrophoresis; Chemistry; Protein kinase A; Substrate (aquarium); Intracellular; Green fluorescent protein; Phosphorylation; Dephosphorylation; Fluorescence; Kinase; Biochemistry; C2C12; Biophysics; Molecular biology; Chromatography; In vitro; Biology; Phosphatase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006068844,0.0006341022,0.0003380245,0.0005033226,0.0003343956,0.0006491356,0.0006919467,0.0008982355,0.0005694035],"category_scores_gemma":[0.000636163,0.0002888093,0.0002385078,0.0003115349,0.0005586019,0.0006173618,0.0004028601,0.001075888,0.0004428799],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004883992,"about_ca_system_score_gemma":0.0005199244,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005966529,"about_ca_topic_score_gemma":0.0008578125,"domain_scores_codex":[0.9994019,0.0001235533,0.0000355732,0.0001964053,0.0001766551,0.00006580291],"domain_scores_gemma":[0.999311,0.0002729046,0.0001102486,0.00007360632,0.0001433646,0.00008907622],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003066129,0.00001364335,0.0001157917,0.00004978784,0.000005521836,0.00005250526,0.00001797098,0.00004985027,0.9971107,0.0003414928,0.00003278071,0.002179324],"study_design_scores_gemma":[0.000006846693,0.00006647928,0.0004379845,0.000004948203,0.000007052473,0.0003746358,0.00001121864,0.00137788,0.9962533,0.0000880668,0.001364059,0.000007497566],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5717078,0.009037305,0.4125032,0.0005400576,0.0002387395,0.0001340635,0.0002902414,0.0009637664,0.004584939],"genre_scores_gemma":[0.7008759,0.004514104,0.2893389,0.0002266355,0.00006312422,0.0002431664,0.0005543699,0.0001501333,0.004033669],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008982355,"threshold_uncertainty_score":0.003543615,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0177350704550619,"score_gpt":0.234568323808449,"score_spread":0.2168332533533871,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}