{"id":"W2023467195","doi":"10.1021/bi0120897","title":"Proximity of Bound Hoechst 33342 to the ATPase Catalytic Sites Places the Drug Binding Site of P-glycoprotein within the Cytoplasmic Membrane Leaflet","year":2002,"lang":"en","type":"article","venue":"Biochemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":80,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Förster resonance energy transfer; Chemistry; Binding site; Fluorophore; P-glycoprotein; A-site; ATP hydrolysis; ATPase; Acceptor; Biophysics; Fluorescence; Fluorescence anisotropy; Substrate (aquarium); Biochemistry; Membrane; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002744842,0.0004060717,0.0002567887,0.0002621158,0.000522134,0.0003412404,0.0003609175,0.0004579712,0.00255383],"category_scores_gemma":[0.0003720375,0.000322506,0.0001871394,0.0003537012,0.0004832388,0.0005470364,0.0002891126,0.001052836,0.0004970441],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005038489,"about_ca_system_score_gemma":0.0004483329,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003052122,"about_ca_topic_score_gemma":0.004100599,"domain_scores_codex":[0.999751,0.00003552172,0.000009286051,0.00008250442,0.00007012096,0.0000515154],"domain_scores_gemma":[0.9997428,0.0001259724,0.00003642933,0.00002571443,0.00003595255,0.00003299072],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000243102,0.00001380167,0.0001672902,0.00003141879,0.000004424305,0.00005440932,0.00003058283,0.0001090378,0.9980987,0.0004277019,0.0000395097,0.0009989875],"study_design_scores_gemma":[0.000009452674,0.00006321028,0.002806071,0.000006873039,0.000008055715,0.0001369003,0.00003081346,0.002003396,0.9927945,0.0002300297,0.001903472,0.000007188115],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9668037,0.001960909,0.02603653,0.0001914273,0.00003889675,0.00003032611,0.000205712,0.000148603,0.004583946],"genre_scores_gemma":[0.9664661,0.001182832,0.02688565,0.00007784212,0.00001343287,0.00007433275,0.0004566823,0.0000534899,0.004789535],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003052122,"threshold_uncertainty_score":0.008543372,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01333581625199489,"score_gpt":0.2209732163867436,"score_spread":0.2076374001347487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}