{"id":"W2023467195","doi":"10.1021/bi0120897","title":"Proximity of Bound Hoechst 33342 to the ATPase Catalytic Sites Places the Drug Binding Site of P-glycoprotein within the Cytoplasmic Membrane Leaflet","year":2002,"lang":"en","type":"article","venue":"Biochemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":80,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Förster resonance energy transfer; Chemistry; Binding site; Fluorophore; P-glycoprotein; A-site; ATP hydrolysis; ATPase; Acceptor; Biophysics; Fluorescence; Fluorescence anisotropy; Substrate (aquarium); Biochemistry; Membrane; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005651401,0.0002243575,0.0003242119,0.00003508406,0.0002122142,0.00002390219,0.0003904523,0.0000856609,0.0001028333],"category_scores_gemma":[0.0001204864,0.0001062054,0.0001560997,0.0003630464,0.0003411581,0.00003935691,0.00005701958,0.0002987755,0.00003493869],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004397538,"about_ca_system_score_gemma":0.0000787594,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008076837,"about_ca_topic_score_gemma":0.0001245846,"domain_scores_codex":[0.998521,0.00003182006,0.0004118361,0.0002947436,0.0004786458,0.0002619823],"domain_scores_gemma":[0.9986261,0.0001220543,0.000249745,0.0008301164,0.00008674564,0.00008522382],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002074888,0.0001363408,0.001006923,0.0006242838,0.0000876277,0.00001632101,0.001926772,0.00002281921,0.9936201,0.00003325808,0.002190615,0.0001274277],"study_design_scores_gemma":[0.0006091817,0.00004167181,0.0002386657,0.0003234144,0.0001718482,0.00003416396,0.001268128,0.0002865555,0.9961914,0.00002050733,0.0006809888,0.0001334175],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9926603,0.0012871,0.00001700831,0.004160587,0.00009812488,0.0007273083,0.0001049222,0.0000340017,0.0009105949],"genre_scores_gemma":[0.9903008,0.00004584238,0.0001603894,0.0001905182,0.0001322797,0.00004716091,0.00004653475,0.00002580546,0.009050612],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008140017,"threshold_uncertainty_score":0.433093,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01333581625199489,"score_gpt":0.2209732163867436,"score_spread":0.2076374001347487,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}