{"id":"W2024057352","doi":"10.1016/j.bej.2010.07.017","title":"Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: A review","year":2010,"lang":"en","type":"review","venue":"Biochemical Engineering Journal","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":272,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Ottawa","funders":"Ontario Ministry of Research and Innovation; Natural Sciences and Engineering Research Council of Canada; University of Ottawa","keywords":"Pichia pastoris; Bioprocess; Alcohol oxidase; Heterologous; Biochemical engineering; Biotechnology; Yeast; Synthetic biology; Protease; Fermentation; Biology; Computational biology; Recombinant DNA; Biochemistry; Engineering; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006823979,0.001386094,0.001861286,0.001896785,0.0002603497,0.001234469,0.001337795,0.001032642,0.00173463],"category_scores_gemma":[0.000500474,0.000443487,0.0005607992,0.003147644,0.0003930132,0.001603023,0.0006224248,0.001180879,0.001884261],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006318568,"about_ca_system_score_gemma":0.000987352,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009410088,"about_ca_topic_score_gemma":0.001444081,"domain_scores_codex":[0.9997848,0.00001772473,0.00003850479,0.00004467537,0.00009387173,0.00002042771],"domain_scores_gemma":[0.9997268,0.00009970815,0.00005592971,0.00001058233,0.00007849249,0.00002842875],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"design_other","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001213752,0.0001058841,0.0001479091,0.02358184,0.0001148261,0.0002748119,0.00004226254,0.0006082676,0.015242,0.001405304,0.0130972,0.9452583],"study_design_scores_gemma":[0.00004026599,0.000220077,0.001151726,0.002786261,0.0003379433,0.001619014,0.00006076372,0.0002539245,0.01089443,0.001123342,0.9814568,0.00005560672],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"review","genre_gemma":"review","genre_scores_codex":[0.0003928712,0.9979497,0.0006829474,0.0001171098,0.0001736106,0.000008336442,0.00004004225,0.0000166093,0.0006187882],"genre_scores_gemma":[0.0007836031,0.9977435,0.000687058,0.00008519749,0.00007882132,0.000007510385,0.00007342458,0.000002998734,0.000537834],"genre_candidate":"review","genre_consensus":"review","teacher_disagreement_score":0.001896785,"threshold_uncertainty_score":0.005802989,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01351866022225401,"score_gpt":0.2788628971229924,"score_spread":0.2653442369007384,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}