{"id":"W2024305637","doi":"10.1002/1615-9861(200208)2:8<978::aid-prot978>3.0.co;2-3","title":"Proteomic analysis of human biopsy samples by single two-dimensional electrophoresis: Coomassie, silver, mass spectrometry, and Western blotting","year":2002,"lang":"en","type":"article","venue":"PROTEOMICS","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University","funders":"","keywords":"Blot; Coomassie Brilliant Blue; Silver stain; Two-dimensional gel electrophoresis; Staining; Gel electrophoresis; Mass spectrometry; Proteomics; Molecular biology; Chemistry; Peptide mass fingerprinting; Polyacrylamide gel electrophoresis; Chromatography; Biology; Biochemistry; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001205637,0.001320751,0.0009663196,0.00209286,0.0008595753,0.0008739029,0.0008162775,0.0008316724,0.0008719097],"category_scores_gemma":[0.0009309492,0.0004577067,0.0006786929,0.001196793,0.0007101857,0.0006871232,0.0005912153,0.0009606159,0.001328332],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000267872,"about_ca_system_score_gemma":0.0004844851,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006539635,"about_ca_topic_score_gemma":0.001799383,"domain_scores_codex":[0.9991446,0.0001641796,0.000125454,0.0002468938,0.0002466078,0.00007238858],"domain_scores_gemma":[0.9993966,0.0001241335,0.00008942364,0.0001126212,0.0002118163,0.00006543534],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008001284,0.00004114869,0.0003396866,0.0001866181,0.00001994719,0.00009958776,0.00003627296,0.00004057821,0.99518,0.0001026251,0.0001268612,0.003746778],"study_design_scores_gemma":[0.0000307224,0.0003572567,0.01137476,0.00004553286,0.00008835712,0.001899793,0.0001234404,0.003177654,0.9732882,0.0004551499,0.009121988,0.00003705766],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3595486,0.0162263,0.6082816,0.0007803709,0.0007804765,0.001895688,0.004184191,0.003223766,0.005079082],"genre_scores_gemma":[0.2728366,0.01923027,0.6922116,0.0005378555,0.0001990441,0.002356959,0.006247663,0.0006477407,0.005732208],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.00209286,"threshold_uncertainty_score":0.006376088,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01868519492389024,"score_gpt":0.2620862330455228,"score_spread":0.2434010381216326,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}