{"id":"W2024430051","doi":"10.1039/c4cc05418k","title":"Direct enzyme–substrate affinity determination by real-time hyperpolarized<sup>13</sup>C-MRS","year":2014,"lang":"en","type":"article","venue":"Chemical Communications","topic":"Advanced NMR Techniques and Applications","field":"Chemistry","cited_by":3,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"Israel Science Foundation","keywords":"Choline oxidase; Metabolite; Chemistry; Choline; Substrate (aquarium); Enzyme; Nuclear magnetic resonance; Analytical Chemistry (journal); Chromatography; Biochemistry; Physics; Biology; Acetylcholinesterase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008593979,0.001234703,0.001013398,0.0003123843,0.0002670501,0.0009535712,0.0007903038,0.0008959088,0.001686222],"category_scores_gemma":[0.001730599,0.0006631558,0.0002284416,0.0003991971,0.0005570645,0.0006767071,0.0003629255,0.0009713156,0.002021064],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004429595,"about_ca_system_score_gemma":0.0006420173,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001618665,"about_ca_topic_score_gemma":0.002754874,"domain_scores_codex":[0.9993667,0.000131988,0.00003966548,0.0002351323,0.0001433458,0.00008314801],"domain_scores_gemma":[0.9990256,0.0003720535,0.0001190616,0.0001429601,0.0002808304,0.00005944083],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001509601,0.00001154535,0.0003127264,0.00006539268,0.00001334568,0.00004058853,0.00001550805,0.0001324809,0.9946216,0.0001192901,0.0001089429,0.00440754],"study_design_scores_gemma":[0.000005829252,0.00006478355,0.001427468,0.000003168817,0.00002688008,0.0002118824,0.00001423919,0.003141963,0.9934877,0.0001065279,0.001496847,0.00001269882],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5470524,0.008970181,0.4316284,0.000541558,0.0001618004,0.0003010765,0.001897035,0.002275029,0.00717258],"genre_scores_gemma":[0.7981132,0.004488249,0.1878629,0.0003540817,0.00006679839,0.0002725155,0.001607031,0.0005753438,0.006659854],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001686222,"threshold_uncertainty_score":0.005640924,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01871333994204502,"score_gpt":0.2832116480532876,"score_spread":0.2644983081112426,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}