{"id":"W2024638835","doi":"10.1016/j.pep.2008.06.004","title":"Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)—characterization of its phosphatase activity and interaction with Golli-BG21","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; University of California, Los Angeles","keywords":"Chemistry; Circular dichroism; Recombinant DNA; Myelin basic protein; Phosphatase; Alkaline phosphatase; Biochemistry; Gene isoform; Fast protein liquid chromatography; Molecular biology; Stereochemistry; Enzyme; Myelin; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002789419,0.0001722845,0.0002138236,0.00009445589,0.0001793571,0.0000171605,0.0001153864,0.0001454231,0.00001669981],"category_scores_gemma":[0.0002367867,0.0001225069,0.00003565904,0.0001672612,0.0001577903,0.00008856504,0.000117993,0.0001648856,3.736708e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001453319,"about_ca_system_score_gemma":0.00006643168,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005533253,"about_ca_topic_score_gemma":0.000005502277,"domain_scores_codex":[0.9985594,0.0002616864,0.0003744633,0.000399067,0.0002685194,0.0001368783],"domain_scores_gemma":[0.9984831,0.0000274637,0.0007498302,0.0003600223,0.000307315,0.00007231894],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00144748,0.0002115053,0.0005685604,0.000293078,0.00001487981,5.855467e-7,0.0003905355,0.000004958876,0.9864107,0.00007634268,0.000006990347,0.01057435],"study_design_scores_gemma":[0.0008769394,0.0003002407,0.02720635,0.0005801307,0.00001064673,0.00002225956,0.0001005683,0.000503867,0.9696782,0.00001923762,0.000575866,0.0001256629],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.995424,0.0002799947,0.002312449,0.0002013583,0.00004164481,0.001640993,0.00003964475,0.000007519441,0.0000523866],"genre_scores_gemma":[0.9977056,0.0005268743,0.001040366,0.000004870387,0.00002441187,0.0002097811,0.0001086711,0.00001776146,0.0003616254],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02663779,"threshold_uncertainty_score":0.4995688,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01646363913382189,"score_gpt":0.2584390961954424,"score_spread":0.2419754570616205,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}