{"id":"W2024638835","doi":"10.1016/j.pep.2008.06.004","title":"Expression and purification of the active variant of recombinant murine Golli-interacting protein (GIP)—characterization of its phosphatase activity and interaction with Golli-BG21","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Guelph","funders":"Natural Sciences and Engineering Research Council of Canada; University of California, Los Angeles","keywords":"Chemistry; Circular dichroism; Recombinant DNA; Myelin basic protein; Phosphatase; Alkaline phosphatase; Biochemistry; Gene isoform; Fast protein liquid chromatography; Molecular biology; Stereochemistry; Enzyme; Myelin; Biology; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002869129,0.0005521317,0.0003366941,0.0003898623,0.0002640527,0.0005213699,0.0007830047,0.0003588272,0.0007143135],"category_scores_gemma":[0.0003502826,0.0002573573,0.0004328041,0.0006538295,0.0002777965,0.0003885748,0.0003533052,0.001120444,0.0008400464],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008093624,"about_ca_system_score_gemma":0.0003337595,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001536492,"about_ca_topic_score_gemma":0.001123857,"domain_scores_codex":[0.9998247,0.00002313783,0.00001782906,0.00003285135,0.00005007298,0.00005144369],"domain_scores_gemma":[0.9997905,0.00003803352,0.00004356914,0.00004492907,0.00003166019,0.0000512445],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008871139,0.00003680593,0.0002404626,0.00002542873,0.000008668718,0.00007076879,0.00002299277,0.00008255235,0.9984913,0.0002262986,0.00007121509,0.0006347453],"study_design_scores_gemma":[0.00001994793,0.00007278343,0.004478416,0.000004473983,0.00002116523,0.0004131969,0.00003023314,0.001396894,0.9897859,0.00009836203,0.003668966,0.00000959007],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9834388,0.001256387,0.01101877,0.0003135102,0.00004652103,0.0000857328,0.002573389,0.00014036,0.001126481],"genre_scores_gemma":[0.9580891,0.00120431,0.01784755,0.0001314653,0.00003805185,0.0001384072,0.0169474,0.0001294083,0.005474458],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001536492,"threshold_uncertainty_score":0.005872309,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01646363913382189,"score_gpt":0.2584390961954424,"score_spread":0.2419754570616205,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}