{"id":"W2024793354","doi":"10.1016/j.pep.2010.03.015","title":"Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins","year":2010,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"S100 Proteins and Annexins","field":"Biochemistry, Genetics and Molecular Biology","cited_by":25,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University","funders":"Canadian Institutes of Health Research","keywords":"Escherichia coli; Chemistry; Codon usage bias; Start codon; Microbiology; Molecular biology; Biochemistry; Biology; Gene; Base sequence; Genome","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002402945,0.0001663141,0.0001596674,0.00005311305,0.0002226575,0.00003733174,0.0001151429,0.0002562167,0.00003657181],"category_scores_gemma":[0.0001260225,0.0001458523,0.00004287549,0.00006119929,0.0001109202,0.00001989761,0.00006878001,0.0001143371,5.081098e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003220527,"about_ca_system_score_gemma":0.00003058803,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001231848,"about_ca_topic_score_gemma":0.000007174673,"domain_scores_codex":[0.9989704,0.00005540601,0.0002803903,0.0004132367,0.0001181072,0.0001624826],"domain_scores_gemma":[0.9991666,0.000009591567,0.0002493659,0.0003190661,0.0001609853,0.0000943647],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001535235,0.00009905844,0.00006637714,0.0001125118,0.000004186031,5.626228e-8,0.00009897546,0.00001756944,0.9964072,0.0004393268,0.00007753628,0.002523727],"study_design_scores_gemma":[0.0007873259,0.0003620878,0.0003562511,0.00009969259,0.000007628135,8.080773e-7,0.00007060095,0.0004190907,0.9947327,0.000225477,0.002754611,0.0001836847],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9379221,0.0002525206,0.05970222,0.00008975474,0.00004855579,0.001722148,0.00002198129,0.00002115059,0.0002195579],"genre_scores_gemma":[0.9548957,0.00006583577,0.04341323,0.00002845962,0.0001326352,0.0007794744,0.0001834054,0.00002712687,0.0004741326],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01697359,"threshold_uncertainty_score":0.5947683,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01066971197560447,"score_gpt":0.2596179981506194,"score_spread":0.2489482861750149,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}