{"id":"W2025042523","doi":"10.1038/nprot.2006.215","title":"Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation","year":2006,"lang":"en","type":"article","venue":"Protocol Exchange","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":65,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Neurosphere; Cryopreservation; Neural stem cell; Stem cell; Biology; Cell biology; Adult stem cell; Endothelial stem cell; Embryo; Biochemistry; In vitro","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001150103,0.0001472166,0.0001338354,0.00004903224,0.0001170859,0.00009338867,0.0001455257,0.0001327642,0.00005470034],"category_scores_gemma":[0.000005880943,0.0001303363,0.00007553881,0.0002675741,0.00005002127,0.000009524686,0.000153772,0.00008607726,0.000007632029],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001227524,"about_ca_system_score_gemma":0.00001948296,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009466555,"about_ca_topic_score_gemma":0.0002741347,"domain_scores_codex":[0.9988315,0.0001126609,0.0001956547,0.0004202448,0.0001939575,0.0002459469],"domain_scores_gemma":[0.9994666,0.000004056153,0.00007096346,0.0002788236,0.0001087112,0.00007084956],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004775228,0.00008259204,0.03195523,0.0001059404,0.00002694114,0.000003847174,0.00001994339,0.0001091913,0.9569919,0.000006056523,0.009995887,0.0006547854],"study_design_scores_gemma":[0.001170687,0.0003314583,0.03072719,0.00000405377,0.00005834308,0.000005084899,0.00001053414,0.006561944,0.8280098,0.00001478207,0.1328374,0.0002686649],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8854452,0.0004525276,0.006062963,0.0003316008,0.00006444313,0.1056709,0.00004674643,0.00004563534,0.001879949],"genre_scores_gemma":[0.7284968,0.00003354962,0.00154398,0.0003832404,0.001104837,0.2459537,0.0005476835,0.00005426134,0.02188186],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1569484,"threshold_uncertainty_score":0.5314959,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01871394148115271,"score_gpt":0.2833254818627765,"score_spread":0.2646115403816237,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}