{"id":"W2025042523","doi":"10.1038/nprot.2006.215","title":"Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation","year":2006,"lang":"en","type":"article","venue":"Protocol Exchange","topic":"Pluripotent Stem Cells Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":65,"is_retracted":false,"has_abstract":false,"ca_institutions":"McMaster University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Neurosphere; Cryopreservation; Neural stem cell; Stem cell; Biology; Cell biology; Adult stem cell; Endothelial stem cell; Embryo; Biochemistry; In vitro","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001349508,0.001857728,0.0013664,0.002605591,0.001994255,0.001100734,0.002066547,0.0007198814,0.01030609],"category_scores_gemma":[0.001116024,0.0006596027,0.0006753104,0.002581929,0.00104959,0.001294893,0.001259199,0.001726962,0.006161489],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008289847,"about_ca_system_score_gemma":0.001671839,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002413706,"about_ca_topic_score_gemma":0.006504701,"domain_scores_codex":[0.999164,0.00008696148,0.0001723959,0.000163366,0.0003310116,0.00008230505],"domain_scores_gemma":[0.9990566,0.0002183318,0.00007283306,0.0002738354,0.0003055448,0.00007274552],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.000211847,0.0001299343,0.0004807792,0.0003711349,0.00002342506,0.0003631596,0.000270542,0.000369974,0.9650873,0.002490168,0.002082161,0.0281196],"study_design_scores_gemma":[0.00003279503,0.0001157921,0.002363273,0.00003687694,0.00006879854,0.0006222147,0.00005182786,0.000679459,0.9589173,0.0008502712,0.03622708,0.00003429673],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"protocol","genre_scores_codex":[0.2299214,0.006603239,0.6550534,0.001454707,0.001298944,0.01456986,0.01810876,0.005687526,0.06730206],"genre_scores_gemma":[0.1826162,0.009886121,0.698364,0.0006082945,0.0002817322,0.01780273,0.01813323,0.003673884,0.0686338],"genre_candidate":"protocol","genre_consensus":null,"teacher_disagreement_score":0.01030609,"threshold_uncertainty_score":0.03447729,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01871394148115271,"score_gpt":0.2833254818627765,"score_spread":0.2646115403816237,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}