{"id":"W2025465262","doi":"10.1016/s0008-6215(01)00108-2","title":"The identification of the catalytic nucleophiles of two β-galactosidases from glycoside hydrolase family 35","year":2001,"lang":"en","type":"article","venue":"Carbohydrate Research","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"Università degli Studi di Camerino","keywords":"Bacillus circulans; Glycoside hydrolase; Chemistry; Glycosidic bond; Galactosidases; Hydrolase; Nucleophile; Stereochemistry; Glycosyl; Glycoside; Enzyme; Hydrolysis; Biochemistry; Glycosylation; Catalysis; Beta-galactosidase","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001105592,0.0002290025,0.0003332589,0.0000867194,0.0004737114,0.0001162289,0.001355029,0.0001619932,0.0001375234],"category_scores_gemma":[0.001115876,0.0001533552,0.0002740994,0.0008874011,0.001032059,0.0001207006,0.0003476435,0.0005495963,0.00002905124],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001446164,"about_ca_system_score_gemma":0.0002410415,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001684303,"about_ca_topic_score_gemma":0.0001190599,"domain_scores_codex":[0.9968012,0.0002228332,0.0008574048,0.0004625715,0.001100511,0.0005555102],"domain_scores_gemma":[0.9953083,0.001933675,0.0003559623,0.001830711,0.0004344005,0.0001369914],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001086407,0.0001095623,0.002142736,0.0000800099,0.00009172502,0.00001214249,0.000240027,0.00006743977,0.9955677,0.0001598373,0.0002012013,0.001218915],"study_design_scores_gemma":[0.0003438934,0.000008669851,0.0009297439,0.000155121,0.00005626187,0.000003733616,0.0007619848,0.001680038,0.9927353,0.00250406,0.0006704041,0.0001508253],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9888343,0.001221264,0.000003646982,0.0004139907,0.0001012769,0.0001612793,0.0001946866,0.00003238366,0.009037206],"genre_scores_gemma":[0.997722,0.00064035,0.000004329003,0.000009792334,0.0002006147,0.0000624318,0.00004007239,0.00004152719,0.001278825],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008887792,"threshold_uncertainty_score":0.6253644,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.04695491809397453,"score_gpt":0.3161168124233206,"score_spread":0.2691618943293461,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}