{"id":"W2025899813","doi":"10.1128/jb.185.17.5287-5289.2003","title":"Efflux of Cytoplasmically Acting Antibiotics from Gram-Negative Bacteria: Periplasmic Substrate Capture by Multicomponent Efflux Pumps Inferred from Their Cooperative Action with Single-Component Transporters","year":2003,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Antibiotic Resistance in Bacteria","field":"Biochemistry, Genetics and Molecular Biology","cited_by":14,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"","keywords":"Efflux; Periplasmic space; Biology; Bacteria; Cytosol; Membrane transport protein; Gram-negative bacteria; Permease; Transporter; Antibiotics; Biochemistry; Multidrug Resistance-Associated Proteins; Microbiology; ATP-binding cassette transporter; Escherichia coli; Enzyme; Gene; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002391418,0.0004828338,0.0006069955,0.0002826408,0.0002422885,0.0007812365,0.0003474101,0.0007340019,0.0004077315],"category_scores_gemma":[0.0005467922,0.0003123757,0.0004633618,0.0002394763,0.0003842653,0.000830948,0.0005738075,0.0005461386,0.0002168875],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004141906,"about_ca_system_score_gemma":0.0002338534,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004878409,"about_ca_topic_score_gemma":0.0005705813,"domain_scores_codex":[0.9998661,0.00001947218,0.00001221162,0.00003073125,0.00004849587,0.00002297905],"domain_scores_gemma":[0.9997643,0.0000854343,0.00004917678,0.00004186375,0.00003096924,0.00002824674],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001262291,0.00002410176,0.001821444,0.00007576565,0.00001208415,0.0003879085,0.00003690003,0.002259606,0.9889711,0.002244647,0.00004226955,0.003998045],"study_design_scores_gemma":[0.00004081353,0.0002725545,0.01072972,0.00002333745,0.00009871738,0.001903725,0.0001303786,0.1173576,0.8642716,0.003891398,0.001222362,0.00005787401],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9828388,0.0009943121,0.01536729,0.0001346583,0.00001282547,0.00000761823,0.00002260721,0.00007143937,0.0005504393],"genre_scores_gemma":[0.9957807,0.0003913502,0.003450866,0.00001951188,0.000004997135,0.000005354517,0.0000290209,0.000009354666,0.0003088651],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007812365,"threshold_uncertainty_score":0.003005207,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01425665298453505,"score_gpt":0.2274025318795725,"score_spread":0.2131458788950374,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}