{"id":"W2025988318","doi":"10.1371/journal.pone.0005185","title":"In Vivo and In Vitro Protein Ligation by Naturally Occurring and Engineered Split DnaE Inteins","year":2009,"lang":"en","type":"article","venue":"PLoS ONE","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":80,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Natural Sciences and Engineering Research Council of Canada; Academy of Finland","keywords":"Intein; Protein splicing; Trans-splicing; RNA splicing; Context (archaeology); Protein tag; Ligation; Chemical biology; Protein engineering; Biology; Computational biology; Biochemistry; Chemistry; Gene; Molecular biology; Recombinant DNA; RNA; Fusion protein; Enzyme","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006582964,0.0004956243,0.0002849507,0.0001658214,0.000120111,0.000405181,0.000348143,0.0003083484,0.0003172701],"category_scores_gemma":[0.0004588552,0.0001884556,0.0003962836,0.0002030096,0.0004005595,0.0003859308,0.0002689555,0.0006412663,0.0002087666],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005114397,"about_ca_system_score_gemma":0.0002062486,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004324237,"about_ca_topic_score_gemma":0.0003917868,"domain_scores_codex":[0.9995345,0.0001452168,0.00005067495,0.00009860924,0.0001115257,0.00005958312],"domain_scores_gemma":[0.9997069,0.0001015094,0.00008783452,0.00004059907,0.00003050975,0.00003254704],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009160705,0.00002974057,0.000427633,0.00006959526,0.0000120752,0.00002999716,0.00002259632,0.0006455995,0.9972287,0.0003618704,0.00001652503,0.001063959],"study_design_scores_gemma":[0.000004217,0.00007427896,0.0005470559,0.000002348057,0.00001027063,0.0000908862,0.000009491174,0.003756891,0.9949432,0.00005779943,0.0004995793,0.000003882345],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9775954,0.00104056,0.02050648,0.00004454721,0.00001367489,0.00001878589,0.0001405367,0.00006759081,0.0005724971],"genre_scores_gemma":[0.9900739,0.0004347613,0.008395006,0.0000168363,0.000004466143,0.00001694852,0.0004148244,0.00002689417,0.0006163016],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006582964,"threshold_uncertainty_score":0.003710747,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008702017670002955,"score_gpt":0.1983320685704932,"score_spread":0.1896300509004902,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}