{"id":"W2026073993","doi":"10.1016/j.jneumeth.2005.03.008","title":"Exposure of nuclear antigens in formalin-fixed, paraffin-embedded necropsy human spinal cord tissue: Detection of NeuN","year":2005,"lang":"en","type":"article","venue":"Journal of Neuroscience Methods","topic":"RNA Interference and Gene Delivery","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":false,"ca_institutions":"Western University; Robarts Clinical Trials","funders":"Canadian Institutes of Health Research; Alzheimer Society","keywords":"NeuN; Immunohistochemistry; Immunofluorescence; Immunostaining; Antigen retrieval; Spinal cord; Antigen; Biology; Pathology; Staining; Molecular biology; Primary and secondary antibodies; Antibody; Medicine; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008927168,0.0001193702,0.0002741992,0.0001776213,0.00005209461,0.00001590743,0.0003876379,0.00009825936,0.00000728121],"category_scores_gemma":[0.0002041232,0.0001052137,0.0001314916,0.0002426949,0.0001613597,0.00003122479,0.0000761581,0.0001784223,5.921699e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001120284,"about_ca_system_score_gemma":0.00005841478,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000009553748,"about_ca_topic_score_gemma":0.00001332509,"domain_scores_codex":[0.9985262,0.0002256338,0.0006478989,0.0001885522,0.0002126879,0.000198983],"domain_scores_gemma":[0.9989259,0.00001345616,0.0005854303,0.000215156,0.0001884845,0.00007158666],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003367052,0.00009395837,0.0003907952,0.00001336229,0.000004150634,0.000007238082,0.00006263286,0.0002719546,0.9412841,0.00001281514,0.00002411143,0.05749819],"study_design_scores_gemma":[0.0003701478,0.005823826,0.02220061,0.00003465895,0.00001236269,0.0001657873,0.00008613049,0.0002303167,0.96787,0.00003146643,0.003080084,0.00009462499],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9850018,0.0002784973,0.01418161,0.00007037395,0.000327848,0.000065477,0.00000207456,0.000002012044,0.00007029098],"genre_scores_gemma":[0.9769911,0.0001832247,0.02248929,0.0001566024,0.0001224669,6.407224e-7,3.513873e-7,0.00001006554,0.00004623948],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.05740357,"threshold_uncertainty_score":0.4290492,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03763661033339021,"score_gpt":0.3931564437364962,"score_spread":0.355519833403106,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}