{"id":"W2026242260","doi":"10.1021/bi992744z","title":"Nucleotide-Induced Conformational Changes in P-glycoprotein and in Nucleotide Binding Site Mutants Monitored by Trypsin Sensitivity","year":2000,"lang":"en","type":"article","venue":"Biochemistry","topic":"Drug Transport and Resistance Mechanisms","field":"Medicine","cited_by":42,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"","keywords":"Vanadate; Trypsin; Nucleotide; Mutant; Biochemistry; Chemistry; Cyclic nucleotide-binding domain; ATP hydrolysis; Walker motifs; Molecular biology; Cleavage (geology); Biology; Enzyme; ATPase; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000218747,0.0001885452,0.0002882364,0.00008002036,0.00004669168,0.00002011848,0.00004138229,0.0001834053,0.0001859291],"category_scores_gemma":[0.00001978527,0.0001864441,0.00003465588,0.0002056374,0.00005516408,0.00007391616,0.00001077643,0.0002313985,0.00002688322],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00009543962,"about_ca_system_score_gemma":0.0000574281,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001001641,"about_ca_topic_score_gemma":0.0001088464,"domain_scores_codex":[0.9988983,0.00001925669,0.000261214,0.0002859829,0.0002311242,0.0003041782],"domain_scores_gemma":[0.9996091,0.00002263441,0.00005592779,0.0001611198,0.00002267873,0.0001285972],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002986096,0.0001019227,0.009857443,0.0001566685,0.00001003677,0.0002191033,0.0001976105,0.000001165255,0.9844245,0.000001773544,0.00008968885,0.004641464],"study_design_scores_gemma":[0.002113725,0.00003188834,0.04186844,0.0004829972,0.00001399303,0.00004927222,0.0002910906,0.0003470539,0.9541978,0.00001006504,0.0003624671,0.000231206],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976054,0.0001446355,0.00000283771,0.0005349906,0.00003842676,0.000270615,0.0001066077,0.00004326011,0.001253163],"genre_scores_gemma":[0.9982342,0.00009389128,0.0002175912,0.0001092837,0.00006813517,0.00001487284,0.0001825807,0.00002048389,0.001059004],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.032011,"threshold_uncertainty_score":0.7602969,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007901082408294643,"score_gpt":0.228901375022406,"score_spread":0.2210002926141113,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}