{"id":"W2027563558","doi":"10.1006/prep.2000.1380","title":"Molecular Cloning, Expression, Purification, and Characterization of Fructose-1,6-bisphosphate Aldolase from Thermus aquaticus","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Cancer, Hypoxia, and Metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":41,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal; Université du Québec à Montréal","funders":"","keywords":"Thermus aquaticus; Aldolase A; Thermus; Cloning (programming); Molecular cloning; Fructose-bisphosphate aldolase; Fructose 1,6-bisphosphatase; Biochemistry; Aldolase B; Chemistry; Biology; Molecular biology; Computational biology; Fructose; Enzyme; Gene expression; Gene; Polymerase; Computer science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003896117,0.0007912152,0.0007281253,0.00050821,0.0008427989,0.0005757739,0.0007068333,0.0004430121,0.001003124],"category_scores_gemma":[0.0005050835,0.0004582467,0.0005671373,0.001036881,0.0003633619,0.00046015,0.0003690657,0.001209907,0.0009996988],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007714189,"about_ca_system_score_gemma":0.0008217072,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.005447309,"about_ca_topic_score_gemma":0.005401098,"domain_scores_codex":[0.9997025,0.0000398517,0.0000356461,0.00005032834,0.0001051944,0.00006643836],"domain_scores_gemma":[0.99972,0.00006001245,0.00004063768,0.00003780176,0.0000717775,0.00006976175],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002139834,0.0001064278,0.0003042029,0.0001822303,0.00001516693,0.0001446557,0.0001371887,0.0003222323,0.9941613,0.000576512,0.0004722459,0.003363839],"study_design_scores_gemma":[0.00006159574,0.0002577286,0.01130653,0.00003618252,0.00004169796,0.0006755933,0.0001638318,0.002968888,0.9488888,0.000544211,0.03500739,0.00004743491],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9368207,0.00416948,0.03708029,0.001782968,0.000386032,0.0006217968,0.01427856,0.0006101548,0.004250053],"genre_scores_gemma":[0.7975516,0.00549451,0.09310394,0.0004025336,0.0001779913,0.0006729639,0.07206176,0.0004810126,0.03005368],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.005447309,"threshold_uncertainty_score":0.01083118,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007881224647111688,"score_gpt":0.2268904036214649,"score_spread":0.2190091789743532,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}