{"id":"W2027926561","doi":"10.1074/jbc.m110.163691","title":"Topological Orientation of Acyl-CoA:Diacylglycerol Acyltransferase-1 (DGAT1) and Identification of a Putative Active Site Histidine and the Role of the N Terminus in Dimer/Tetramer Formation","year":2010,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":152,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Canadian Institutes of Health Research","keywords":"Endoplasmic reticulum; Diacylglycerol kinase; Acyltransferase; Transmembrane domain; Biochemistry; Tetramer; Membrane topology; Active site; Myristoylation; Biology; Enzyme; Chemistry; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001096044,0.0003360701,0.0001483666,0.0001959161,0.0001549567,0.000281309,0.0001995428,0.000272221,0.0006047857],"category_scores_gemma":[0.0001603318,0.0001446224,0.0002469381,0.000182347,0.000178995,0.0002559635,0.0001670786,0.0004277128,0.0003700606],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003369004,"about_ca_system_score_gemma":0.0001636946,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004755457,"about_ca_topic_score_gemma":0.0003853607,"domain_scores_codex":[0.9999307,0.00001286857,0.000006248469,0.00002476825,0.00001495697,0.00001051442],"domain_scores_gemma":[0.9999033,0.00001125369,0.00003483432,0.000009001685,0.0000171557,0.00002444216],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001375928,0.00001325176,0.001080267,0.00003843311,0.000003811454,0.00005604156,0.00002167748,0.0002880422,0.9965464,0.0001928617,0.00003187831,0.001589714],"study_design_scores_gemma":[0.00001906157,0.0001515007,0.01901143,0.000008300346,0.00002822797,0.0007420704,0.00006214186,0.007264545,0.9690002,0.0002083932,0.003488906,0.00001529579],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9941618,0.0009420386,0.003946537,0.00009298261,0.00001553285,0.00001651552,0.0003170492,0.0000394335,0.0004681134],"genre_scores_gemma":[0.9906439,0.0007411866,0.00565804,0.00003167731,0.000009239359,0.00001351231,0.001673534,0.0000161535,0.001212741],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006047857,"threshold_uncertainty_score":0.002444386,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00497445528652749,"score_gpt":0.2244044794972055,"score_spread":0.2194300242106781,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}