{"id":"W2027926561","doi":"10.1074/jbc.m110.163691","title":"Topological Orientation of Acyl-CoA:Diacylglycerol Acyltransferase-1 (DGAT1) and Identification of a Putative Active Site Histidine and the Role of the N Terminus in Dimer/Tetramer Formation","year":2010,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Lipid metabolism and biosynthesis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":152,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Saskatchewan","funders":"Canadian Institutes of Health Research","keywords":"Endoplasmic reticulum; Diacylglycerol kinase; Acyltransferase; Transmembrane domain; Biochemistry; Tetramer; Membrane topology; Active site; Myristoylation; Biology; Enzyme; Chemistry; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004359247,0.0000953209,0.0002566746,0.00001989723,0.00003071413,0.000006052126,0.0001458971,0.0001743277,0.000007377951],"category_scores_gemma":[0.0006010312,0.00004694948,0.00009575005,0.00007842245,0.0005740324,0.00001475427,0.0000534062,0.0001817327,5.825285e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004784737,"about_ca_system_score_gemma":0.00003001048,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000007179375,"about_ca_topic_score_gemma":0.000002336403,"domain_scores_codex":[0.9990627,0.00009455384,0.0005255688,0.0001171491,0.0001170428,0.00008298646],"domain_scores_gemma":[0.9990067,0.0001228464,0.0005687028,0.0001200535,0.0001490944,0.00003254582],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0006946641,0.0000831941,0.006626792,0.00002857008,0.00002560232,2.972988e-7,0.0003595506,0.00000162365,0.9870644,0.00003021976,0.000001585932,0.005083516],"study_design_scores_gemma":[0.0007970404,0.00007841212,0.05140718,0.00001305099,0.00003880807,0.00003337273,0.0005170301,0.00002670849,0.9466156,0.0002896865,0.0001331533,0.00004999834],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9991215,0.0004323054,0.00006346934,0.000132805,0.00004543928,0.0001122638,0.00004473196,8.269944e-7,0.00004663702],"genre_scores_gemma":[0.9994612,0.0002769044,0.0001451538,0.00001773181,0.00007143017,0.000003947474,0.00001398186,0.000002825195,0.000006867834],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04478039,"threshold_uncertainty_score":0.2115046,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00497445528652749,"score_gpt":0.2244044794972055,"score_spread":0.2194300242106781,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}