{"id":"W2028286002","doi":"10.1006/bbrc.1999.1950","title":"A Cysteine-Free Firefly Luciferase Retains Luminescence Activity","year":2000,"lang":"en","type":"article","venue":"Biochemical and Biophysical Research Communications","topic":"bioluminescence and chemiluminescence research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Toronto","funders":"Medical Research Council","keywords":"Luciferase; Cysteine; Bioluminescence; Mutant; Luciferin; Firefly protocol; Biochemistry; Chemistry; Serine; Enzyme; Biology","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0007544804,0.0008368547,0.0007801199,0.0005024158,0.0004971417,0.001186552,0.000947992,0.0008687252,0.001873756],"category_scores_gemma":[0.0006184177,0.0003598178,0.000835339,0.000603138,0.0006325346,0.0008052138,0.0003857719,0.001594449,0.001743106],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001339893,"about_ca_system_score_gemma":0.0008565848,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001676561,"about_ca_topic_score_gemma":0.001625111,"domain_scores_codex":[0.9995521,0.00005146901,0.00004791614,0.0001178212,0.0001155009,0.0001152194],"domain_scores_gemma":[0.9990212,0.0002413628,0.00009189678,0.0002344481,0.0001859612,0.0002251539],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000121213,0.00005917799,0.0001859696,0.00004695699,0.00001167136,0.00006113131,0.00002792492,0.00003384317,0.9977991,0.0003414863,0.0001392186,0.00117227],"study_design_scores_gemma":[0.0000116096,0.00005585711,0.0009872101,0.000003873977,0.00001210399,0.0002076961,0.00001265573,0.0002715109,0.9951827,0.00006766128,0.00317902,0.000008021748],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9574488,0.00249436,0.03057912,0.0005975488,0.0004583379,0.0001057351,0.002848118,0.001364632,0.004103333],"genre_scores_gemma":[0.9413658,0.001436028,0.01967271,0.000380425,0.0001025869,0.0001271704,0.01620644,0.0004711676,0.02023765],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001873756,"threshold_uncertainty_score":0.009721696,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.05665792642255753,"score_gpt":0.3666981083329103,"score_spread":0.3100401819103528,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}