{"id":"W2028576752","doi":"10.1042/bj20050060","title":"Functional reconstitution of the <i>Salmonella typhimurium</i> PhoQ histidine kinase sensor in proteoliposomes","year":2005,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Kindai University; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Histidine kinase; Dephosphorylation; Response regulator; Histidine; Biochemistry; Phosphorylation; Autophosphorylation; Kinase; Extracellular; Two-component regulatory system; Biology; Transmembrane protein; Chemistry; Protein kinase A; Cell biology; Enzyme; Phosphatase; Gene; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000152232,0.0001157072,0.0001245507,0.00004511244,0.00005101226,0.00001110427,0.000191008,0.0002675864,0.0000443081],"category_scores_gemma":[0.0001009114,0.0000826331,0.0001064434,0.0001104664,0.0002139211,0.000002757695,0.0001019762,0.0002296992,0.000005414918],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003421777,"about_ca_system_score_gemma":0.000118663,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000379053,"about_ca_topic_score_gemma":0.000006347572,"domain_scores_codex":[0.9991667,0.00004099304,0.0003051422,0.0001817064,0.0001175194,0.0001879944],"domain_scores_gemma":[0.999513,0.000005459218,0.0001412391,0.0001959448,0.00008670502,0.00005762604],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00013879,0.00009595006,0.001230648,0.00000627376,0.00001496723,0.000001485354,0.000003519872,0.00003079284,0.9933492,0.00005095084,0.003465438,0.001611965],"study_design_scores_gemma":[0.0005806363,0.00007922582,0.002831522,0.00002438307,0.000009768281,0.000245385,0.000007827892,0.00001515621,0.9655107,0.00006882677,0.03052255,0.0001040475],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964297,0.001017187,0.0001098198,0.00177371,0.0003094554,0.0001206111,0.00001285958,0.00000430452,0.0002222961],"genre_scores_gemma":[0.9973047,0.0002608366,0.001288738,0.000166587,0.0007568954,0.000004827373,0.00001340293,0.000008742971,0.0001952031],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02783854,"threshold_uncertainty_score":0.336968,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009407214381300294,"score_gpt":0.2093087430381951,"score_spread":0.1999015286568948,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}