{"id":"W2028576752","doi":"10.1042/bj20050060","title":"Functional reconstitution of the <i>Salmonella typhimurium</i> PhoQ histidine kinase sensor in proteoliposomes","year":2005,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Kindai University; Natural Sciences and Engineering Research Council of Canada; McGill University","keywords":"Histidine kinase; Dephosphorylation; Response regulator; Histidine; Biochemistry; Phosphorylation; Autophosphorylation; Kinase; Extracellular; Two-component regulatory system; Biology; Transmembrane protein; Chemistry; Protein kinase A; Cell biology; Enzyme; Phosphatase; Gene; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004080847,0.000700591,0.0003910683,0.0001807513,0.0001850639,0.0005414697,0.0006429676,0.0005285192,0.0008117987],"category_scores_gemma":[0.0002394639,0.0002831074,0.0005184297,0.0002313853,0.000315237,0.0004781963,0.0005513532,0.0008545553,0.0008235619],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007445872,"about_ca_system_score_gemma":0.0003189718,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001574459,"about_ca_topic_score_gemma":0.0008371626,"domain_scores_codex":[0.9997775,0.00004771966,0.00003666401,0.00004686537,0.0000412405,0.00004999151],"domain_scores_gemma":[0.999836,0.00002998435,0.00004371622,0.00002785482,0.00002386752,0.0000386274],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001121624,0.00004070212,0.00009595601,0.00006807994,0.00001391599,0.00004199867,0.00003465579,0.0001820595,0.9985864,0.0003545629,0.00005288471,0.0004167123],"study_design_scores_gemma":[0.00003601644,0.0001066962,0.0007697776,0.000009156804,0.00002468466,0.00008080308,0.00003123757,0.001411476,0.9949649,0.00007591146,0.002478329,0.00001099423],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9761583,0.001028725,0.0182584,0.0003656814,0.0001157751,0.000150063,0.001996143,0.000358881,0.001568026],"genre_scores_gemma":[0.9860503,0.0007041555,0.007635772,0.00006859204,0.00001567585,0.0001117056,0.002629311,0.0001203459,0.002664156],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001574459,"threshold_uncertainty_score":0.005402327,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009407214381300294,"score_gpt":0.2093087430381951,"score_spread":0.1999015286568948,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}