{"id":"W2029702664","doi":"10.1152/ajprenal.00082.2004","title":"Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na<sup>+</sup>/H<sup>+</sup> exchanger NHE3 at PKA consensus sites","year":2005,"lang":"en","type":"article","venue":"American Journal of Physiology-Renal Physiology","topic":"Ion Transport and Channel Regulation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":121,"is_retracted":false,"has_abstract":true,"ca_institutions":"McGill University","funders":"National Institute of Diabetes and Digestive and Kidney Diseases","keywords":"Phosphorylation; Serine; Molecular biology; Polyclonal antibodies; Chemistry; In vivo; Kinase; Western blot; Protein phosphorylation; Endogeny; Protein kinase A; Biology; Antibody; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001150917,0.002204831,0.001135672,0.0009655425,0.001103439,0.0007850201,0.001308022,0.001189184,0.008613088],"category_scores_gemma":[0.0004166013,0.000977349,0.001535942,0.0009008599,0.0007183614,0.0009783966,0.0007470755,0.003876037,0.001480435],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001047209,"about_ca_system_score_gemma":0.0007987634,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009337995,"about_ca_topic_score_gemma":0.002694842,"domain_scores_codex":[0.9987407,0.0001120139,0.0002150552,0.0004355705,0.0002560094,0.0002406631],"domain_scores_gemma":[0.9992494,0.0002204335,0.0001367719,0.00009765709,0.0001711022,0.0001246996],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001147535,0.0001127406,0.0001281068,0.0002027745,0.00003091687,0.00004447756,0.00003902497,0.00004290143,0.9976236,0.000268202,0.0001091905,0.001283304],"study_design_scores_gemma":[0.0001644688,0.0008810171,0.005335717,0.00004975171,0.0002058267,0.0009726032,0.00008536948,0.002073157,0.9815236,0.0003091086,0.008375343,0.00002409523],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7951515,0.01224858,0.169103,0.001379141,0.001233722,0.001448386,0.003165584,0.00109097,0.01517907],"genre_scores_gemma":[0.6345566,0.01789393,0.2980427,0.001571498,0.0005421806,0.006215488,0.01455384,0.0005363446,0.02608743],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.008613088,"threshold_uncertainty_score":0.0288136,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02528172877995929,"score_gpt":0.2361585620801958,"score_spread":0.2108768333002365,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}