{"id":"W2029711985","doi":"10.1074/jbc.m400451200","title":"Intermediate Trapping on a Mutant Retaining α-Galactosyltransferase Identifies an Unexpected Aspartate Residue","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":99,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC","keywords":"Chemistry; Galactosyltransferase; Stereochemistry; Population; Hydrolase; Glycosyl; Nucleophile; Carboxylate; Residue (chemistry); Glycosyltransferase; Active site; Uridine; Galactose; Enzyme; Biochemistry; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008006518,0.0003842188,0.0002175938,0.00008329858,0.0001007021,0.0002669346,0.0003393163,0.0003114274,0.0008429933],"category_scores_gemma":[0.0001741206,0.000107729,0.0002813766,0.0001341295,0.0002398737,0.0001248123,0.0001880336,0.0004231157,0.0005409218],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003129337,"about_ca_system_score_gemma":0.0001748615,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001321299,"about_ca_topic_score_gemma":0.0008461718,"domain_scores_codex":[0.9999207,0.0000120562,0.00001064393,0.00001729966,0.0000235032,0.00001577035],"domain_scores_gemma":[0.9998726,0.00002894682,0.00004511315,0.00001262964,0.00001378714,0.00002697544],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007934867,0.00002402367,0.0001929785,0.00001071022,0.000005236153,0.0001759766,0.00001081514,0.00007938947,0.9987279,0.00007698031,0.00001827606,0.000598433],"study_design_scores_gemma":[0.00001777319,0.0001672492,0.002481775,0.000004428239,0.00001229018,0.0006840111,0.00002484634,0.001551268,0.9940633,0.00003443741,0.0009522201,0.000006329346],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.996519,0.00009437981,0.002563674,0.000049594,0.00001251049,0.00001663918,0.0002843876,0.00005191987,0.0004079548],"genre_scores_gemma":[0.9951673,0.0001205428,0.002943531,0.00003407791,0.00000323684,0.00001219171,0.0005737894,0.00005102155,0.001094362],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001321299,"threshold_uncertainty_score":0.002820075,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03497643615251322,"score_gpt":0.257428528292653,"score_spread":0.2224520921401398,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}