{"id":"W2029711985","doi":"10.1074/jbc.m400451200","title":"Intermediate Trapping on a Mutant Retaining α-Galactosyltransferase Identifies an Unexpected Aspartate Residue","year":2004,"lang":"en","type":"article","venue":"Journal of Biological Chemistry","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":99,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC","keywords":"Chemistry; Galactosyltransferase; Stereochemistry; Population; Hydrolase; Glycosyl; Nucleophile; Carboxylate; Residue (chemistry); Glycosyltransferase; Active site; Uridine; Galactose; Enzyme; Biochemistry; Catalysis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow","insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0003919198,0.0003704727,0.0006124279,0.00004604312,0.0001350449,0.0001055567,0.0005447431,0.0004355214,0.001145577],"category_scores_gemma":[0.0007014577,0.000281597,0.0003966859,0.0001739485,0.0002353936,0.0001696112,0.00003973648,0.000930584,0.00000749019],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001915609,"about_ca_system_score_gemma":0.0001162656,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005977522,"about_ca_topic_score_gemma":0.000001528496,"domain_scores_codex":[0.9977597,0.0000393113,0.0009635353,0.000412871,0.0003736851,0.0004509252],"domain_scores_gemma":[0.9983616,0.0002398538,0.000476991,0.0003691056,0.0001675198,0.0003849318],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000675734,0.0002964528,0.0003629267,0.0001122655,0.0001171081,0.0008076919,0.0003586155,0.0001669208,0.9965073,0.000007630498,0.0000138643,0.0005734413],"study_design_scores_gemma":[0.001228747,0.0001318724,0.0003056632,0.0004777101,0.00005343771,0.0003846033,0.001202155,0.00001514955,0.9949959,0.0007262988,0.0001323835,0.0003461039],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9942772,0.0001443231,0.00004741759,0.000238389,0.00007577432,0.00002808822,0.00003287021,0.00008068054,0.005075211],"genre_scores_gemma":[0.9988728,0.0001785629,0.00006699842,0.00007785529,0.0006360783,0.000005751535,0.00003558097,0.00002581773,0.0001005118],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004974699,"threshold_uncertainty_score":0.9999636,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03497643615251322,"score_gpt":0.257428528292653,"score_spread":0.2224520921401398,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}