{"id":"W2030350092","doi":"10.1006/prep.2001.1521","title":"Production of an Anti-Prostate-Specific Antigen Single-Chain Antibody Fragment from Pichia pastoris","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Pichia pastoris; Antibody; Antigen; Monoclonal antibody; Molecular biology; Prostate-specific antigen; Ammonium sulfate precipitation; Biology; Pichia; Epitope; Chemistry; Recombinant DNA; Prostate cancer; Biochemistry; Gene; Enzyme; Size-exclusion chromatography; Cancer; Immunology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003170046,0.0009440036,0.0009822677,0.0006013978,0.0003940152,0.0007340778,0.001054002,0.0005086139,0.001562589],"category_scores_gemma":[0.0002286765,0.0005744951,0.0007110379,0.000909727,0.0002570175,0.0003338254,0.0004721754,0.001752246,0.001547834],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000900781,"about_ca_system_score_gemma":0.0004863279,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003789284,"about_ca_topic_score_gemma":0.003001326,"domain_scores_codex":[0.9997714,0.00002194619,0.00003006929,0.00004754688,0.00007678791,0.000052259],"domain_scores_gemma":[0.999836,0.00003750922,0.00001938165,0.00003149515,0.00002742917,0.00004813992],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009819688,0.00004497046,0.0001352234,0.000115234,0.00002705771,0.00009379068,0.00002721285,0.0001863156,0.9968793,0.0001812157,0.0002765422,0.001934856],"study_design_scores_gemma":[0.00007918353,0.0001691231,0.003317462,0.00002513985,0.0001285012,0.0003423049,0.00002736256,0.003211356,0.9767042,0.0001144203,0.01585763,0.00002328826],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8885615,0.005177957,0.07149066,0.001095906,0.001099575,0.000629313,0.01812542,0.00212213,0.01169751],"genre_scores_gemma":[0.8507435,0.005212427,0.07469409,0.000478868,0.0001470552,0.000439319,0.03900087,0.0004641677,0.02881964],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003789284,"threshold_uncertainty_score":0.007534444,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03369662283253135,"score_gpt":0.2994908699655321,"score_spread":0.2657942471330008,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}