{"id":"W2030350092","doi":"10.1006/prep.2001.1521","title":"Production of an Anti-Prostate-Specific Antigen Single-Chain Antibody Fragment from Pichia pastoris","year":2001,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"","keywords":"Pichia pastoris; Antibody; Antigen; Monoclonal antibody; Molecular biology; Prostate-specific antigen; Ammonium sulfate precipitation; Biology; Pichia; Epitope; Chemistry; Recombinant DNA; Prostate cancer; Biochemistry; Gene; Enzyme; Size-exclusion chromatography; Cancer; Immunology; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002958187,0.0001759327,0.0002761207,0.0001492999,0.0001708456,0.00003257998,0.00009797765,0.0001154498,0.0002068067],"category_scores_gemma":[0.00005185015,0.0001374471,0.00005206538,0.0002108493,0.0001215803,0.0002045623,0.00004428233,0.0001914908,0.00001815065],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002752164,"about_ca_system_score_gemma":0.00003255305,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000177981,"about_ca_topic_score_gemma":0.000003343504,"domain_scores_codex":[0.9982734,0.0001360872,0.0003857531,0.0004902399,0.0004815854,0.0002329857],"domain_scores_gemma":[0.99901,0.0000245029,0.0001781976,0.0004114544,0.0001856095,0.0001902123],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009408547,0.0005430513,0.01734476,0.00008001526,0.0000153313,0.000009872629,0.0006107454,0.000003552984,0.9431451,0.0000909133,0.0002203464,0.03699547],"study_design_scores_gemma":[0.0006598091,0.0005020093,0.1550931,0.0003636888,0.00001158331,0.00001609074,0.000288283,0.0002207802,0.7955468,0.0003713478,0.04675879,0.0001676375],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9937476,0.002312161,0.0006215482,0.001655039,0.0001603166,0.0010716,0.00002305259,0.0000537387,0.0003549416],"genre_scores_gemma":[0.9908701,0.00175027,0.005346327,0.00003911256,0.0003932576,0.00006000773,0.0004655932,0.0000225456,0.001052834],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1475982,"threshold_uncertainty_score":0.5604932,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03369662283253135,"score_gpt":0.2994908699655321,"score_spread":0.2657942471330008,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}